Food and Fermentation Industries >

2014 , Vol. 40 >Issue 06: 8 - 12

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.2014.06.013

Food and Fermentation Industries

Optimization of permeability of E. coli cells in the production of trehalose synthase by a visualized method

  • CUI Huai-yan ,
  • JIANG Ling ,
  • ZHANG Wen-jing ,
  • TANG Fei ,
  • XU Xian ,
  • LI Shuang ,
  • HUANG He
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Online published: 2014-06-25

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Abstract

For optimizing the technological conditions of producing trehalose synthase from E. coli BL21 after permeated by colistin sulfate,a novel visualized method for the operation of industrial process called visualized optimization method was adapted in this paper. The results indicated that a proper process was achieved with cell suspension treated by 1. 40 g /L colistin sulfate for 72 min at 35℃ and the obtained enzyme activity was 2. 09 times higher than that from traditional method. Utilizing the permeabilized cells for batch fermentation,the maximum conversion rate could reach 70% with 30% maltose as the substrate. The cells could be also used for at least 16 batches( 12 h per batch),and the conversion rate kept above 65%.

Key words： trehalose synthase; colistin sulfate; permeable treatment; visualized optimization method

Cite this article

CUI Huai-yan , JIANG Ling , ZHANG Wen-jing , TANG Fei , XU Xian , LI Shuang , HUANG He . Optimization of permeability of E. coli cells in the production of trehalose synthase by a visualized method[J]. Food and Fermentation Industries, 2014 , 40(06) : 8 -12 . DOI: 10.13995/j.cnki.11-1802/ts.2014.06.013

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