The dynamic adsorption conditions of AB-8 macroporous resin,such as concentration of resveratrol,work speed of the infusion and pH,were investigated using response surface methodology on basis of single factor experiment. The model of a second order quadratic equation for the adsorption rate of AB-8 macroporous resin was established to optimize the adsorption process. Then,desorption conditions of AB-8 macroporousresinAB-8 were also discussed. The results indicated that concentration of resveratrol,work speed of the infusion and pH had a significant effect on the adsorption rate. The optimal parameters were 7. 85mg /mL resveratrol concentration,work speed of the infusion of 1. 43 BV /hand,pH of 4. 11 and the adsorption rate was 95. 03%. The purity of resveratrol was 46. 92%when eluting 0. 9 BV with 75% ethanol for 1 hour.