Capabilities in inhibiting cholesterol oxidation of southern wild grape wines caused by free radical in vitro were studied using a cholesterol oxidation emulsion system similar to healthy body similar conditions,and the content of the main antioxidants in wines were analyzed. The results showed that Gui-Pu No. 1 and brier grape wines could inhibit cholesterol oxidation obviously. The inhibition ability of Gui-Pu No. 1 wine was higher than brier grape wine. When wines were added into the cholesterol oxidation emulsion system at a ratio of 1∶ 50,1∶ 100,1∶ 200( v /v),the inhibition ratio of Gui-pu No. 1 after 24 h reaction were 98. 4%,97. 2%,80. 9%,respectively,and those of the brier grape wine were 96. 6%,92. 9%,72. 8%. The contents of free anthocyanins and proanthocyanidin in Gui-Pu No. 1 and brier grape wine reached 759. 9,403. 1mg /L and 712. 6,465. 0 mg /L respectively,and the contents of resveratrol were 5. 62 mg /L and 4. 25 mg /L. Rich polyphenol antioxidants in southern wild grape wines were the material basis to inhibit cholesterol oxidation.