To study the effect of the content of selenium on coenzyme Q content in plants,Se-rich soybean was produced via Foliar applications of selenite. The content of selenium in soybean was determined by atomic fluorescence spectrometry,and content of coenzyme Q in soybean was determined by high performance liquid chromatography. The results showed that Se content was significantly increased in Se fertilizer sprayed soybean,from 78. 89 μg /kg to 345. 12 μg /kg. The effect of different concentrations of Selenium on the content of coenzyme Q10 in soybean was also studied. The result indicated that the content of coenzyme Q10 increased observably in Se-rich soybean. A linear relationship between the content of coenzyme Q10 and Se was discovered. The linear equation was y = 0. 0504 x +2. 3748. Therefore,Se-enriched treatment can rise the content of coenzyme Q10 in soybean. Therefore increase the nutrition of soybean.