Because of the significant difference between the content of SO2 in Kiwi wine and that in fermented mash measured by oxidation method,we put forward the hypothesis that the Vc of Kiwi wine or fermented may interfere with the determination of SO2 by iodine titration.This experiment confirmed that the solution concentration of Vc and the standard solution volume of I2 had a significant positive correlation,and the regression equation was Y =87.604 X-30.197,R2= 0.9996.Vc has the similar reducibility with SO2,so Vc competitively consumed the standard solution of I2 with SO2,which caused the artificially high results.The both results of SO2 in Kiwi wine or fermented mash measured by oxidation and iodine titration were significantly different(P < 0.01).Based on the experimental results,it was recommended that the content of SO2 of the Kiwi wine or fermented mash was measured by oxidation.Meanwhile,the paper summarized the researches on the determination method of SO2 and the usage advices from some scholars,which could provide the reference for the measurement of SO2 in samples such as Kiwi wine to reduce the result differences or even wrong judgments due to improper method.