Co-precipitation method was used to prepare the inclusion complexes of ethyl butyrate with β-and γ-cyclodextrin.The optimal inclusion conditions were determined by orthogonal array design experiments.The results showed that the inclusion ratio of β-cyclodextrin complex could reach to 46.69 % when ethyl butyrate /β-cyclodextrin molecular ratio was 1∶ 1,ultrasound time was 30 min and ultrasound temperature was 50 ℃.The inclusion ratio of γ-cyclodextrin complex could reach 95.59 % when ethyl butyrate /γ-cyclodextrin molecular ratio was 1∶ 1,ultrasound time was 45 min and ultrasound temperature was 60 ℃.The interactions between ethyl butyrate and cyclodextrins were analyzed by scanning electron microscope(SEM),infrared spectrometry(IR) and X-ray diffraction(XRD).The complexes formation was confirmed,and γ-cyclodextrin was more suitable for wall material of ethyl butyrate.