The objective of this study was to investigate the antifungal activities of sugar fatty acid esters against food spoilage fungi strains.Sugar fatty acid esters were synthesized by immobilized lipase in acetone in the presence of molecular sieve and further purified by slica-gel column chromatography.The susceptibility of four strains of fungi(Penicilium oxalicum,Aspergillus tubingensis,Mucor circinelloides,Rhizopus nigricans) to different sugar fatty acid esters at different pH values were evaluated.Fructose monolaurate had the highest antifungal activity against Penicilium oxalicum and Aspergillus tubingensis.The antifungal activity enhanced with increasing concentration of fructose monolaurate.Fructose monolaurate affected not only the size,but also the thickness and pigment production of mold colony.The pH value had a significant impact on antifungal activity.The antifungal activity of fructose monolaurate under neutral conditions was lower than that under acidic and alkaline conditions.