After immersion 6h in 10 mg /L Na2SeO3 solution,soybean was germinated 96 h at optimal conditions.Soybean protein isolate(SPI) was obtained from germinated soybean with alkaline extraction and acid precipitation method.After adding GDL,soy protein gels were analyzed by Texture Analyzer and the impact of Se and the germination time on gel property was investigated.The results showed that the hardness of soybean gel and SPI gel decreased rapidly from 25.25 g to 10.77 g and from 27.73 g to 13.37 g respectively during 48 h germination.Water holding capacity(WHC) of soybean gel and SPI gel decreased from 61.42% to 51.55% and from 62.64% to 55.54% respectively.The changes of SPI during germination were discussed by SDS-PAGE mapping.The results showed that β-7S subunit and B-11 S subunit were relatively stable,while α'-7S,α-7S,A3-11 S and A-11 S subunits were easily hydrolyzed by intra-proteases.At the same germination time,the protein bands of se-enriched soybean were similar with the control group.It showed that enriched selenium treatment had a little effect on gel property of soybean protein during germination.