The optimal composition of culture solution for γ-aminobutyric acid( GABA) under hypoxia was Na Cl42. 49 mmol / L,VB662. 6 μmol / L and Ca Cl26. 21 mmol / L. GABA content in soybean sprouts was 2. 77 mg / g DW under this condition. The changes of physiological and biochemical parameters of soybean germinated under hypoxia indicated that the growth and the respiration was inhibited,the consumption of crude protein increased. The free amino acid content increased,the glutamate decarboxylase( GAD) and diamine oxidase( DAO) activity was enhanced,thus GABA content was enriched.