Inulin fructotransferase was firstly immobilized using sodium alginate and gelatin as entrapment carriers. The effects of several factors such as concentrations of gelatin,sodium alginate and Ca Cl2 and embedding time were explored. The properties of immobilized enzyme were also investigated. The results indicated that the enzyme were best immobilized with 20 g / L gelatin,20 g / L sodium alginate,40 g / L Ca Cl2 for 5 h. The enzyme entrapping rate was 95. 6%. The relative enzyme activities were retained over 50% after 8 times of operation. Compared with the free enzyme,the optimum p H value and reaction temperature of immobilized enzyme were 5. 5 ~ 6. 0 and 65 ~ 70 ℃,respectively. It showed that immobilized enzyme had good operation stability.