The poly-glycine modified electrode was prepared using cyclic voltammetry. The electrochemical behavior of allura red on the poly-glycine modified electrode was studied. Anew method to determineallura red was established. The study results showed thatglycine has good catalytic performance to the allura Red. It can be used in the detection of allura red in p H = 3. 0 disodium hydrogen phosphat-citric acid solution. The linear range for the determination of allurared was from 8. 0 × 10-8to 6. 0 × 106 mol / L and the limit of detection was 2. 0 × 10-8mol / L. Some common metal ions and cations had no obvious effects on the determination of allura red. The modified electrodes demonstrated excellent sensitivity,selectivity,and stability. This method was applied to the determination ofallura redin food with satisfactory results.