Rapid and quantitative detection of hop resistance and beer spoilage capability in beer spoilage bacteria can effectively guide the beer manufacture of breweries.In this study,a new culture-dependent rapid test method based on hop tetrahydro-iso-α-acid-gradient medium plate was constructed.23 beer spoilage bacteria strains were used to test the accuracy of method.The minimum inhibitory concentrations of tetrahydro-iso-α-acid,a compound of hop bitter acid,were rapidly detected by the method.The growth capabilities in MRS broth contained tetrahydro-iso-α-acid and 12°P beer broth were compared.These results indicate that the method based on hop tetrahydro-iso-α-acidgradient plate quite rapid and simple to quantitatively assay hop resistance of beer spoilage bacteria,meantime,evaluate the beer spoilage capability.
ZHENG Fei-yun
,
NIU Cheng-tuo
,
ZHU Lin-jiang
,
ZHANG Yi-qiu
,
LI Yong-xian
,
LIU Chun-feng
,
WANG Jin-jingg
,
LI Qi
. Quantitative test of tetrahydro-iso-α-acid resistance of beer spoilage bacteria using hoptetrahydro-iso-α-acid-gradient medium[J]. Food and Fermentation Industries, 2017
, 43(10)
: 212
-216
.
DOI: 10.13995/j.cnki.11-1802/ts.014777
