The fruiting bodies of Grifola frondosa were used as raw material to extract proteins by alkali-solution and acid-isolation, salting-out and acetone precipitation, respectively. And the effects of these methods on the physicochemical and functional properties of the extracted proteins were compared. Results showed that the highest protein purity (45.35%) was obtained by alkali-solution and acid-isolation method. Proteins extracted by the three methods were all rich in trace elements, especially Ca and Mg. And the proteins obtained by the alkali-solution and acid-isolation and the salting-out method contained 17 amino acids, while methionine was not present in the proteins extracted by acetone precipitation method. Scanning electron microscope revealed that the proteins extracted from the acetone precipitation were granular, while the proteins extracted from the other two methods were flaky. The functional properties showed that the proteins from alkali-solution and acid-isolation were better than the others in the solubility, water and oil retention, foaming capacity and foam stability, emulsifying property and emulsion stability. This research provides theoretical references for the further development and utilization of Grifola frondosa.
PENG Zhijie
,
DU Jiao
,
ZHANG Yuting
,
GUO Dongdong
,
LEI Jiayu
,
GENG Xueran
,
MENG Junlong
,
CHANG Mingchang
. Effect of different extraction methods on structure and function of Grifola frondosa crude protein[J]. Food and Fermentation Industries, 2020
, 46(21)
: 161
-166
.
DOI: 10.13995/j.cnki.11-1802/ts.023793
[1] 陈少美,薛小兰. 菌草灰树花蛋白质提取工艺优化研究[J]. 福建农业科技,2018(2):30-32.
[2] 崔凤杰, 许泓瑜,舒畅,等.响应曲面法优化灰树花水溶性多糖提取工艺的研究[J].食品科学,2006,27(4):142-147.
[3] 郭家瑞, 王卫国,李磊,等.灰树花研究概述[J].食用菌,2010,32(4):1-2;13.
[4] 吴智艳, 闫训友.灰树花生理活性物质的研究进展[J].北方园艺,2006,19(6):174-175.
[5] BORCHERS A T,STERN J S,HACKMAN R M,et al.Mushrooms,tumors,and Immunity[J].Proceedings of the Society for Experimental Biology & Medicine,1999,221(4):281-293.
[6] TSAI S Y,WENG C C,HUANG S J,et al.Nonvolatile taste components of Grifola frondosa, Morchella esculenta,and Termitomyces albuminosus,Mycelia[J].LWT-Food Science and Technology,2006,39(10):1 066-1 071.
[7] GU C Q,LI J W,CHAO F,et al.Isolation,identification and function of a Novel Anti-HSV-1 protein from Grifola frondosa[J].Antiviral Research,2007,75(3):250-257.
[8] TSAO Y W,KUAN Y C,WANG J L,et al.Characterization of a novel maitake (Grifola frondosa) protein that activates natural killer and dendritic cells and enhances antitumor immunity in mice[J].Journal of Agricultural and Food Chemistry,2013,61(41):9 828-9 838.
[9] 邓德江. 林地栽培灰树花[J].北京农业,2010,22(22):21-21.
[10] 王盼盼. 食品中蛋白质的功能(三)食品中蛋白质的功能特性[J].肉类研究,2009,23(6):62-71.
[11] 徐志宏, 魏振承,张雁,等.几种蛋白质功能性质的比较研究[J].食品科学,2006,27(12):249-252.
[12] 杨颖, 徐代勋,曲勃,等.梅花鹿鹿茸总蛋白的提取方法比较[J].特产研究,2011,33(2):11-12.
[13] 陈雪洋, 刘亚琼,于振林,等.杏鲍菇蛋白质提取及功能性质测定[J].食品科技,2017,42(5):235-240.
[14] 任雨贺,刘淑莹,万茜淋.人参蛋白质不同提取方法及含量的比较[J].安徽农业科学,2018,46(11):1-3;9.
[15] 魏君慧, 薛媛,冯莉,等.杏鲍菇分离蛋白和清蛋白的理化性质及功能分析[J].食品科学,2018,39(18):54-60.
[16] 高燕红, 鲁琳,刘应亮.6种食用菌蛋白质与氨基酸的含量分析及评价[J].现代预防医学,2010,37(10):1 843-1 846;1 849.
[17] 夏克东. 星油藤蛋白的制备及其理化性质的研究[D].郑州:河南工业大学,2016.
[18] 李露敏, 代红发,王报贵,等.樟树籽仁蛋白的组成及功能性质研究[J].食品工业科技,2016,37(3).
[19] 郑文彬. 黑龙江省芸豆主栽品种蛋白质功能性质分析及降血压肽制备研究[D].大庆:黑龙江八一农垦大学,2016.
[20] 郭勇. 酶工程(第四版)[M].北京:科学出版社,2017,85-88.
[21] 张强, 吴彩娥.羊肚菌菌丝体蛋白的理化特性及抗氧化活性[J].浙江农业学报,2016,28(8):1 408-1 415.
[22] 史瑞婕, 郭丰铭,董亚楠,等.杏鲍菇蛋白质功能特性研究[J].食品科技,2018,43(2):131-136.
[23] 郑新雷. 杏鲍菇分离蛋白的制备、理化功能特性与抗氧化活性研究[D].南宁:广西大学,2019.
[24] FAO/WHO.Energy and protein requirements[R].Geneva:World Health Organization,1973.
[25] 李伟. 扫描电子显微镜及其分析技术简介[J].山东电力技术,1996(2):77;38.
