High-density fermentation of Lactobacillus salivarius was conducted under aerobic conditions and the number of viable bacteria was measured.Using total viable count as the indicator to study the optimal conditions for aerobic respiration of L.salivarius,the number of viable bacteria and the survival time were improved for large-scale industrial fermentation.A Lactobacillusstrain named GDW1994 was screened and identified as L.salivarius by 16S rDNA sequencing.The effect of acid and hydrogen peroxide on the growth of strain L.salivarius GDW1994 revealed that the activation of its respiratory chain required the simultaneous addition of heme and tetraene naphthalene (VK2).The optimized medium was peptone 10 g/L,yeast extract 2.5 g/L,glucose 20 g/L,beef paste 15 g/L,anhydrous sodium 2 g/L,MgSO4 0.6 g/L,MnSO4·H2O 0.2 g/L.The optimal conditions were 37℃,inoculation volume 4%,pH 6.4,160 r/min and catalase 30 g/L.With the acquisition of heme and tetraene methanone,and the participation of oxygen,the GDW 1994 strain could be preserved for up to 30 d at 4 ℃.The total viable count reached 108 CFU/mL and survival rate was 100 times higher than that of the medium without heme,tetraene naphthalene and oxygen.It was 20 times higher than that of the culture medium added with exogenous catalase.
ZHOU Lijun
,
YANG Miaomiao
,
WEI Chunyan
,
PAN Changpei
,
HUANG Shihai
. Anti-oxygen stress ability of Lactobacillus salivarius and optimization of its aerobic fermentation conditions[J]. Food and Fermentation Industries, 2020
, 46(22)
: 98
-105
.
DOI: 10.13995/j.cnki.11-1802/ts.022509
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