A dominant strain was isolated and identified from Fuzhuan brick tea.Optimization of the fermentation process of this strain was studied using the DPPH free radical scavenging rate as an indicator,and its antioxidant capacity and antimicrobial activity were also investigated.The results showed that the dominant strain was identified as Eurotium cristatum and designated as Eurotialessp.GT-1.The optimum liquid fermentation process was as follows:Maltose as carbon source,yeast extract as nitrogen source,fermentation time 9 d,liquid volume 13%,inoculation amount 2.2%.Under this condition,the scavenging rate of DPPH free radical reached 93.9% (2.5 mg/mL).It was also found that the IC50 values of O-2· free radical and DPPH free radical scavenging abilities were 0.78 and 10.70 mg/mL,respectively.When the concentration of fermentation extract was 3 mg/mL,the reducing power was 0.98.The fermentation extract of Eurotiales sp.GT-1 exhibited wide-spectrum antimicrobial activities against Staphylococcus aureus, Bacillus subtilis,Pseudomonas aeruginosa and Salmonella enterica,especially Escherichia coli.This study will provide an important theoretical basis for the production and application of Eurotium cristatum.
ZHANG Yue
,
CUI Xuanxuan
,
LIU Yingxue
,
GAI Yongqiang
,
PIAO Meizi
. Isolation,identification,fermentation technology and bioactivity of Eurotium cristatum in Fuzhuan brick tea[J]. Food and Fermentation Industries, 2020
, 46(22)
: 202
-207
.
DOI: 10.13995/j.cnki.11-1802/ts.024328
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