Escherichia coli co-culture was designed for biosynthesis of caffeyl alcohol glucosides from glucose.Three caffeyl alcohol monoglucosides,namely caffeyl alcohol-3-O-glucoside,caffeyl alcohol-9-O-glucoside and caffeyl alcohol-4-O-glucoside,were produced by co-culturing two recombinantE.coli strains.A biosynthetic pathway ofp-coumaryl alcohol was constructed in the high-tyrosine-producingE.coli BTAL-CAD01 by expressing tyrosine ammonialyase,hydroxycinnamate:CoA ligase (4CL),and cinnamyl-CoA reductase fromRhodotorula glutinis,Petroselinum crispum,andArabidopsis thaliana,respectively,together with endogenous alcohol dehydrogenases or aldo-keto reductases.Then,4-hydroxyphenlacetate 3-hydroxylase (HpaBC) fromE.coli and glucosyltransferase UGT73C5 fromA.thaliana were co-expressed in wild-typeE.coliBL21 (DE3),resulting in a recombinant strain BWT-CAD01.Caffeyl alcohol monoglucosides were successfully produced by co-cultivating the recombinant strains BTAL-CAD01 and BWT-CAD01.The highest titer of caffeyl alcohol-4-O-glucoside reached (141.63±3.42) mg/L,with optimized inoculation ratio of 3:1.To our knowledge,this is the first report on heterologous biosynthesis of caffeyl alcohol glucosides,both caffeyl alcohol-3-O-glucoside and caffeyl alcohol-9-O-glucoside are unnatural compounds.This work expands the structural diversity of caffeyl alcohol glucosides,which makes it possible the discovery of new analogues with improved pharmaceutical properties.
WANG Shuai
,
ZHUANG Yibin
,
LIU Hao
,
BI Huiping
,
LIU Tao
. Production of caffeyl alcohol glucosides by mixed fermentation with differentEscherichia coli recombinants[J]. Food and Fermentation Industries, 2020
, 46(23)
: 7
-13
.
DOI: 10.13995/j.cnki.11-1802/ts.024788
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