Food and Fermentation Industries >

2020 , Vol. 46 >Issue 23: 147 - 155

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.024965

Effect of antagonistic yeast combined with water soluble chitosan on postharvest storage quality ofMalus micromalus fruit

  • CHEN Hua ,
  • WANG Jianjun ,
  • WANG Jianwu ,
  • AI Yinting
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  • 1(Department of Life Sciences,Yulin College,Yulin 719000,China);
    2(Department of Energy Engineering,Yulin College,Yulin 719000,China)

Received date: 2020-08-05

  Revised date: 2020-08-12

  Online published: 2020-12-30

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Abstract

The purpose of this study is to explore the antagonistic yeast compound technology for postharvest storage ofMalus micromalus fruit,Botrytis cinerea was inoculated into the fruit ofM.micromalus,andCryptococcus laurentii was used as the antagonistic yeast to determine the optimal concentration of yeast for biocontrol.Then,the effect of antagonistic yeast combined with water-soluble chitosan (WSC) on the storage quality ofM. micromalus fruit at room temperature was studied.The results of the optimal concentration biocontrol test of antagonistic yeast showed that the yeast solution of 105-108 CFU/mL could significantly reduce the incidence and diameter of the disease in the storage process of the fruit (P <0.05),and the bacteriostatic effect was best under the 107 CFU/mL of yeast solution.The storage quality ofM.micromalus fruit showed that the lowest content of malonaldehyde and maximum activity of antioxidant enzyme in the 7.50 g/L WSC composite treatment group,the weight loss rate,decay rate,soluble solids and VC in the 10.00 g/L WSC composite treatment group were better than other groups.From the 9th day of storage,the indexes of 7.50-10.00 g/L WSC treatment group were significantly(P<0.05)better than yeast treatment group,but there was no significant difference between 7.50 g/L and 10.00 g/L WSC treatment group.In order to reduce cost,postharvest storage ofM.micromalus fruit should be treated with 107 CFU/mLC.laurentii combined with 7.50 g/L WSC.

Key words： water soluble chitosan; antagonistic yeast; Malus micromalus fruit; storage quality

Cite this article

CHEN Hua , WANG Jianjun , WANG Jianwu , AI Yinting . Effect of antagonistic yeast combined with water soluble chitosan on postharvest storage quality ofMalus micromalus fruit[J]. Food and Fermentation Industries, 2020 , 46(23) : 147 -155 . DOI: 10.13995/j.cnki.11-1802/ts.024965

References

[1] 曾荣,董华强,林丽超,等.酵母菌对草莓采后生理及贮藏效果的影响[J].食品与发酵工业,2009,35(7):189-191.
ZENG R,DONG H Q,LIN L C,et al.Effects of yeast on physiology and preservation of post harvest strawberry[J].Food and Fermentation Industries,2009,35(7):189-191.
[2] 闫岩,王明力,李岑,等.汉逊德巴利酵母对采后柑橘青霉病的防治及贮藏品质的影响[J].食品与发酵工业,2013,39(8):216-220.
WNAG Y,WANG M L,LI C,et al.Effects ofDebaryomyces hansenii on blue mold and quality of postharvest orange[J].Food and Fermentation Industries,2013,39(8):216-220.
[3] LAI J,CAO X,YU T,et al.Effect ofCryptococcus laurentii on inducing disease resistance in cherry tomato fruit with focus on the expression of defense-related genes[J].Food Chemistry,2018,254:208-216.
[4] WEI Y Y,XU M,WU H L,et al.Defense response of cherry tomato at different maturity stages to combined treatment of hot air andCryptococcus laurentii[J].Postharvest Biology and Technology,2016,117:177-186.
[5] ZHANG Z Q,CHEN J,LI B Q,et al.Influence of oxidative stress on biocontrol activity ofCryptococcus laurentii against blue mold on peach fruit[J].Frontiers in Microbiology,2017,8:1-10.
[6] LI J K,LI H,JI S F,CHEN T,et al.Enhancement of biocontrol efficacy ofCryptococcus laurentii by cinnamic acid againstPenicillium italicum in citrus fruit[J].Postharvest Biology and Technology,2019,149:42-49.
[7] 林丽,田世平,秦国政,等.两种拮抗酵母菌对桃果实贮藏期间主要病害的防治效果[J].中国农业科学,2003(12):1 535-1 539.
LIN L,TIAN S P,QIN G Z,et al.Biocontrol of postharvest diseases in peach fruits using two antagonistic yeasts during storage periods[J].Scientia Agricultura Sinica,2003(12):1 535-1 539.
[8] PEDRO U B R,MONTSERRAT C S,ROSALÍA S V,NORMA A O Á,et al.Biocontrol action mechanisms ofCryptococcus laurentii onColletotrichumgloeosporioides of mango[J].Crop Protection,2014,65:194-201.
[9] LEONARDO G.NAVARTA,JUAN C,et al.Postharvest control of gray mold in apples with lyophilized formulations ofCryptococcus laurentii:The effect of cold stress in the survival and effectiveness of the yeast[J].Food and Bioprocess Technology,2014,7(10):2 962-2 968.
[10] LAI J,CAO X,YU T,et al.Effect ofCryptococcus laurentii on inducing disease resistance in cherry tomato fruit with focus on the expression of defense-related genes[J].Food Chemistry,2018,254:208-216.
[11] 李青云,刘文玉,魏长庆,等.拮抗酵母对北疆冬枣采后病害生防效果的研究[J].食品工业,2015,36(12):72-75.
LI Q Y,LIU W Y,WEI C Q,et al.Antagonistic yeast against disease and effect on quality of postharvest northern winter jujube[J].The Food Industry,2015,36(12):72-75.
[12] ZHANG Z Q,CHEN J,LI B Q,et al.Influence of oxidative stress on biocontrol activity ofCryptococcus laurentiiagainst blue mold on peach fruit[J].Frontiers in Microbiology,2017,8:151.
[13] MENG X H,QIN G Z,TIAN S P.Influences of preharvest sprayingCryptococcus laurentii combined with postharvest chitosan coating on postharvest diseases and quality of table grapes in storage[J].LWT-Food Science and Technology,2009,43(4).596-601.
[14] 谢春晖,位思清,王兆升,等.壳聚糖涂膜保鲜冬枣的研究[J].山东农业大学学报(自然科学版),2010,41(1):45-50.
XIE C H,WEI S Q,WANG Z S,et al.Study on chitosan coationg in storage of Chinese winter jujube[J].Journal of Shandong Agricultural University(Natural Science),2010,41(1):45-50.
[15] 杨涛,原双进,张润光,等.壳聚糖和噻苯咪唑处理对晋枣鲜果贮藏品质的影响[J].食品与发酵工业,2019,45(8):109-114.
YANG T,YUAN S J,ZHANG R G,et al.Effects of chitosan and thiabendazole on the storage quality of fresh Jin jujube[J].Food and Fermentation Industries,2019,45(8):109-114.
[16] 董泽义,谭丽菊,王江涛.壳聚糖保鲜膜研究进展[J].食品与发酵工业,2014,40(6):147-151.
DONG Z Y,TAN L J,WANG J T.The present situation of chitosan film[J].Food and Fermentation Industries,2014,40(6):147-151.
[17] 曹建康,姜微波,赵玉梅.果蔬采后生理生化试验指导[M].北京:中国轻工业出版社,2007:101-142.
CAO J K,JIANG W B,ZHAO Y M.Guidance for postharvest physiological and biochemical tests of fruits and vegetables[M].Beijing:China Light Industry Press,2007:101-142.
[18] 李慧芸,李蒙蒙,余琼,等.纳他霉素结合壳聚糖保鲜对鲜食核桃霉变及采后生理的影响[J].农产品加工,2019(1):23-26.
LI H Y,LI M M,YU Q,et al.Effect of natamycin combined with chitosan on mildew and physiological and of fresh walnut[J].Farm Products Processing,2019(1):23-26.
[19] 毛淑波,朱娜,韦莹莹,等.采前喷施拮抗菌罗伦隐球酵母对草莓保鲜效果的影响[J].食品工业科技,2013,34(4):344-348.
MAO S B.ZHU N,WEI Y Y,et al.Effect of preharvest spraying antagonistic yeastCryptococcus laurentiion the preservation of strawberry[J].Science and Technology of Food Industry,2013,34(4):344-348.
[20] 梁泉峰,池振明.间型假丝酵母菌株对多种水果蔬菜腐败霉菌的拮抗效果和拮抗机制的研究[J].食品与发酵工业,2002,28(1):34-39.
LIANG Q F,CHI Z M.Study on antagonistic effect and mechanism byCandida intermedia for biological control of moulds on vegetables and fruits[J].Food and Fermentation Industries,2002,28(1):34-39.
[21] SHARMA R R,DINESH S,RAJBIR S.Biological control of postharvest diseases of fruits and vegetables by microbial antagonists:A review[J].Biological Control,2009,50(3):205-221.
[22] 刘普,方静凡,程运江,等.生防酵母菌防治果品采后病害机理的研究进展[J].华中农业大报,2013,32(2):134-140.
LIU P,FANG J F,CHENG Y J,et al.Research progress on action mechanism of biocontrol yeast against postharvest fruit pathogen[J].Journal of Huzhong Agricultural University,2013,32(2):134-140.
[23] 徐丹,刘琴.壳聚糖复合涂膜的微观形貌变化及其对红桔的保鲜效果[J].食品与发酵工业,2018,44(2):233-239.
XU D,LIU Q,Morphologies changes of chitosan composite coating and effects on preservation of tangerine[J].Food and Fermentation Industries,2018,44(2):233-239.
[24] 栗子茜,高彦祥.壳聚糖在果蔬涂膜保鲜的应用[J].中国食品添加剂,2018(1):139-145.
LI Z X,GAO Y X.Chitosan-based coating in fruits and vegetables preservation[J].China Food Additives.2018(1):139-145.
[25] 杨涛,原双进,张润光,等.壳聚糖和噻苯咪唑处理对晋枣鲜果贮藏品质的影响[J].食品与发酵工业,2019,45(8):109-114.
YANG T,YUAN S J,ZHANG R G,et al.Effects of chitosan and thiabendazole on the storage quality of fresh Jin jujube[J].Food and Fermentation Industries,2019,45(8):109-114.
[26] MARTINS C J C,LIMA M K S,SILVA R A,et al.Development of biodegradable films based on purple yam starch/chitosan for food application[J].Heliyon,2020,6(4):3 718-3 728.
[27] 李慧妍,秦文,辛松林,等.壳聚糖和1-甲基环丙烯对黄秋葵果实低温贮藏品质及生理的影响[J].食品与发酵工业,2015,41(12):202-207.
LI H Y,QIN W,XIN S L,et al.Effect of chitosan or 1-methylcyclopropene treatment on physiology and quality of okra stored in low temperature[J].Food and Fermentation Industries,2015,41(12):202-207.
[28] 盘柳依,赵显阳,陈明,等.外源茉莉酸甲酯处理对采后猕猴桃果实品质和抗氧化酶活性的影响[J].食品与发酵工业,2019,45(9):190-196.
PAN L Y,ZHAO X Y,CHEN M,et al.Effects of exogenous methyl jasmonate on fruit qualities and antioxidases activities of postharvest kiwifruits[J].Food and Fermentation Industries,2019,45(9):190-196.
[29] WANG F,DENG J,JIAO J Y,et al.The combined effects of carboxymethyl chitosan andCryptococcus laurentii treatment on postharvest blue mold caused by Penicillium italicum in grapefruit fruit[J].Scientia Horticulturae,2019,253:35-41.
[30] 董红兵,朱蝶.不同浓度的壳聚糖对鲜切胡萝卜的保鲜效果[J].武汉商学院学报,2018,32(4):93-96.
DONG H B,ZHU D.On preservation of fresh-cut carrots with chitosan coating of different concentrations[J].Journal of Wuhan Business University,2018,32(4):93-96.
[31] 秦晓杰,高梦,蒋晓玲,等.采前喷施拮抗酵母菌对草莓采后贮藏性能的影响[J].南京农业大学学报,2015,38(1):152-160.
QIN X J,GAO M,JIANG X L,et al.Effect of preharvest spraying antagonistic yeast on the postharvest storability of strawberry[J].Journal of Nanjing Agricultural University,2015,38(1):152-160.
[32] 彭茹,张璐,曾凯芳.膜醭毕赤酵母与壳聚糖复合处理对柑橘果实防御酶及防御物质的影响[J].食品科学,2014,35(10):212-217.
PENG R,ZHANG L,ZENG K F.Effects ofPichia membranefaciens combined with chitosan on defensive enzymes and substances in citrus fruits[J].Food Science,2014,35(10):212-217.
[33] 当赖廷和,何斌源,范航清,等.重金属Cd胁迫对红树蚬的抗氧化酶、消化酶活性和MDA含量的影响[J].生态学报,2011,31(11):3 044-3 053.
DANG L T H,HE B Y,FAN H Q,et al.Effects of cadmium stress on the activities of antioxidant enzymes,digestive enzymes and the membrane lipid peroxidation of the mangrove mud clamGeloina coaxans (Gmelin)[J].Acta Ecologica Sinica,2011,31(11):3 044-3 053.
[34] 刘括,聂国朝,卢蔚莹,等.VC/壳聚糖复合涂膜对荔枝常温保鲜性能的研究[J].玉林师范学院学报,2018,39(2):49-55.
LIU K,NIE G C,LU W Y,et al.Effect of VC/chitosan composite coatings on fresh keeping property of litchi at room temperature[J].Journal of Yulin Normal University,2018,39(2):49-55.
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