Food and Fermentation Industries >

2020 , Vol. 46 >Issue 23: 184 - 192

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.024733

Purification of total flavonoids from bamboo shoots of Pleioblastus amarus with macroporous resin and its anti-inflammation activity

  • YAN Junling ,
  • FAN Yang ,
  • QIN Chuan ,
  • OU Xue ,
  • CHEN Shujuan ,
  • AO Xiaolin
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  • (College of Food Science,Sichuan Agricultural University,Ya'an 625014,China)

Received date: 2020-06-12

  Revised date: 2020-07-05

  Online published: 2020-12-30

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Abstract

The aim of this work is to develop an efficient and economical method for the enrichment of total flavonoids from the shoots ofP.amarus,and to determine its anti-inflammatory activity in vitro.Before the dynamic adsorption and desorption tests,the resin screening,adsorption kinetics,adsorption isotherm and thermodynamics were studied successively.The screening showed that AB-8 resin is the most suitable resin for purifying the total flavonoids of the shoots ofP.amarus.The static experimental data correlated well with the pseudo-second-order kinetics model and Langmuir isotherm model.The adsorption process is spontaneous and exothermic.The optimum enrichment conditions were as follows:for adsorption the total flavonoids concentration,flow rate and volume of sample were 1.55 mg/mL,1 BV/h and 4.2 BV.Respectively,and for desorption the flavonoids-loaded AB-8 resin column was desorbed by 8 BV of 50% ethanol at a rate of 4 BV/h,the recovery yield of total flavonoids was 75.03%,total flavonoids content increased from 2.46% to 15.72%.The total flavonoids of the purified could better inhibit the production of NO.When the total flavonoids concentration reached 1.04 mg/mL,the inhibition rate reached 97.80%(the concentration of NO was 5.60 mol/L),while the concentration of NO in the crude extracts was 13.81 mol/L,inhibition rate was only 73.22%.In summary,the purification method of total flavonoids of the shoots ofP.amarus established in this experiment is effective and feasible.

Key words： Pleioblastus amarus; total flavonoids; macroporous adsorption resin; adsorption kinetics; adsorption thermodynamics; anti-inflammatory activity

Cite this article

YAN Junling , FAN Yang , QIN Chuan , OU Xue , CHEN Shujuan , AO Xiaolin . Purification of total flavonoids from bamboo shoots of Pleioblastus amarus with macroporous resin and its anti-inflammation activity[J]. Food and Fermentation Industries, 2020 , 46(23) : 184 -192 . DOI: 10.13995/j.cnki.11-1802/ts.024733

References

[1] SUN J,TANG F,YUE Y D,et al.Two new compounds from the dry leaves ofPleioblastus amarus(Keng) keng f[J].Journal of Asian Natural Products Research,2014,16(9),930-935.
[2] GAO Q,JIANG H,TANG F,et al.Evaluation of the bitter components of bamboo shoots using a metabolomics approach[J].Food &function,2019,10(1):90-98.
[3] REN Y,MA Y S,ZHANG Z D,et al.Total alkaloids from bamboo shoots and bamboo shoot shells ofPleioblastus amarus (Keng) Keng f.and their anti-inflammatory activities[J].Molecules (Basel,Switzerland),2019,24(15):2 699.
[4] SUN J,ZHANG P,WEI Q,et al.Amarusine A,a new dioxaspiro[4.4]nonane derivative with a butyrolactone ring fromPleioblastus amarus[J].Tetrahedron Letters,2014,55(33):4529-4531.
[5] 吴昊,唐涛浪,刘伟,等.野生椒蒿总黄酮的纯化工艺及抗氧化活性研究[J].食品研究与开发,2019,40(20):86-91.
WU H,TANG T L,LIU W,et al.Purification and antioxidant activity of total flavonoids fromArtemisia dracunculus L.[J].Food Research and Development,2019,40(20):86-91.
[6] 冯靖,彭效明,李翠清,等.大孔树脂纯化银杏叶中总黄酮的工艺研究[J].应用化工,2019,48(10):2 339-2 343;2 349.
FENG J,PENG X M,LI C Q,et al.Purification of total flavonoids from Ginkgo leaves by macroporous resin[J].Applied Chemical Industry,2019,48(10):2 339-2 343;2 349.
[7] 田徽,阮期平,赖恩阳,等.大孔树脂纯化马兰总黄酮树脂吸附特性及工艺的研究[J].食品与发酵工业,2012,38(1):224-229.TIAN H,RUAN Q P,LAI E Y,et al.Studies on adsorption characteristics and purification process of flavonoids fromKalimeris indica (L) Sch.Bip by macroreticular resin[J].Food and Fermentation Industries,2012,38(1):224-229.
[8] 张斌,孙兰萍,伍亚华,等.花生壳总黄酮的大孔树脂吸附动力学研究[J].中国油脂,2017,42(3):122-126.
ZHANG B,SUN L P,WU Y H,et al.Adsorption kinetics of flavonoids from peanut hull by macroporous resin[J].China Oils and Fats,2017,42(3):122-126.
[9] HOU M Y,HU W Z,XIU Y S,et al.Efficient enrichment of total flavonoids from Pteris ensiformisBurm.extracts by macroporous adsorption resins and in vitro evaluation of antioxidant and antiproliferative activities[J].Journal of Chromatography B,2020,1138:121 960.
[10] 张媛,康龙丽,金天博,等.西藏雪莲总黄酮在大孔吸附树脂上的吸附动力学和热力学研究[J].离子交换与吸附,2016,32(4):358-367.
ZHANG Y,KANG L L,JIN T B,et al.Adsorption kinetics and thermodyanmics of total flavonoids from Saussurea involucrataadsorbed on macroporous adsorption resin[J].Ion Exchange and Adsorption,2016,32(4):358-367.
[11] 段庆,唐小丹,郑希,等.新会陈皮提取物中四种黄酮成分含量测定及其抗炎活性研究[J].现代食品,2019(11):156-162.
DUAN Q,TANG X D,ZHENG X,et al.Determination of four flavonoids in extracts of citrus reticulata ‘Chachi' and their anti-inflammatory activity[J].Modern Food,2019(11):156-162.
[12] 徐祖伟,于朝生,窦烁.AB-8大孔树脂对柴胡总黄酮的吸附行为研究[J].化学工程,2013,41(2):33-37.
XU Z W,YU C S,DOU S,et al.Adsorption behavior of AB-8 macroporous resin for total flavonoids in Bupleurum[J].Chemical Engineering,2013,41(2):33-37.
[13] HOU M Y,HU W Z,XIU Z L,et al.Efficient enrichment of total flavonoids from Pteris ensiformis Burm.extracts by macroporous adsorption resins and in vitro evaluation of antioxidant and antiproliferative activities[J].Journal of Chromatography B,2020,1 138:121 960.
[14] 李园园,李洪娟,侯桂革,等.大孔吸附树脂纯化紫菀总黄酮工艺[J].中成药,2019,41(3):501-505.
LI Y Y,LI H J,HOU G G,et al.Purification process for total flavonoids inAsteris radixetRhizoma with macroporous absorption resin[J].Chinese Traditional Patent Medicine,2019,41(3):501-505.
[15] 欧阳玉祝,李雪峰,魏燕,等.D-101大孔树脂对连翘中黄酮类成分的吸附特性研究[J].核农学报,2013,27(10):1 534-1 538.
OUYANG Y Z,LI X F,WEI Y,et al.Adsorption characteristic of D-101 macroporous resin to flavonoid constituent inFructus forsythiae[J].Journal of Nuclear Agricultural Sciences,2013,27(10):1 534-1 538.
[16] 赵腾飞,刘奥,刘良禹,等.红纤维虾海藻黄酮的大孔树脂纯化及抗氧化活性研究[J].中国海洋药物,2018,37(1):62-68.
ZHAO T F,LIU A,LIU L Y,et al.Study on flavonoids purification by macroporous resin and antioxidant activity of flavonoids fromPhyllospadix iwatensis Makino[J].Chinese Journal of Marine Drugs,2018,37(1):62-68.
[17] JIANG H,LI J,CHEN L,et al.Adsorption and desorption of chlorogenic acid by macroporous adsorbent resins during extraction ofEucommia ulmoides leaves[J].Industrial Crops &Products,2020,149:112 336.
[18] TARUN B,LI L,XU Y Q,et al.Ultrasonic-assisted modifications of macroporous resin to improve anthocyanin purification from aPyrus communis var.Starkrimson extract[J].Ultrasonics Sonochemistry,2020,62:104 853.
[19] WU S H,WANG Y Y,GONG G L,et al.Adsorption and desorption properties of macroporous resins for flavonoids from the extract of Chinese wolfberry ( Lycium barbarum L.)[J].Food and Bioproducts Processing,2015,93:148-155.
[20] CHE Z M S,LEE S Y,TEO C Y,et al.Adsorption and desorption properties of total flavonoids from oil palm (Elaeis guineensis Jacq.) mature leaf on macroporous adsorption resins[J].Molecules (Basel,Switzerland),2020,25(4):778.
[21] XI L,MU T,SUN H,Preparative purification of polyphenols from sweet potato (Ipomoea batatas L.) leaves by AB-8 macroporous resins[J].Food Chemistry.2015,172:166-174.
[22] SUN L J,GUO Y R,FU C C,et al.Simultaneous separation and purification of total poly-phenols,chlorogenic acid and phlorizin from thinned young apples[J].Food Chemistry,2013,136:1 022-1 029.
[23] 芦宇,吕长鑫,焦天慧,等.聚酰胺纯化红树莓籽黄酮及其结合胆酸盐能力评价[J].中国食品学报,2019,19(10):172-178.
LU Y,LU C X,JIAO T H,et al.Ability of bile salt-binding of red raspberry seed flavonoids purifuication by polyamide[J].Journal of Chinese Institute of Food Science and Technology,2019,19(10):172-178.
[24] 王翠芳,王特日格乐,杜红喜,等.沙葱总黄酮水洗组分的体外抗炎活性[J].食品科学,2019,40(23):163-169.
WANG C F,WANG T,DU H X,et al.Anti-inflammatory activityin vitro of water-eluted fraction of total flavonoids extracted fromAllium mongolicum Regel[J].Food Science,2019,40(23):163-169.
[25] 黄文静,孙晓春,周瑞,等.响应面法优化黄芪茎叶总黄酮提取工艺及其抗炎活性研究[J].中国现代中药,2018,20(7):871-877.
HUANG W J,SUN X C,ZHOU R,et al.Response surface methodology for optimization of extracting total flavonoids from stems and leaves of Astragalus membranaceus and anti-inflammatory evaluation[J].Modern Chinese Medicine,2018,20(7):871-877.
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