Food and Fermentation Industries >

2021 , Vol. 47 >Issue 1: 21 - 26

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.025545

Isolation and identification of endophytic fungi from Polygonum cuspidatum for polydatin transformation

  • YU Jie ,
  • XU Qinqian ,
  • LI Ziyuan ,
  • LIU Hongyan ,
  • HAO Zaibin ,
  • LI Haiyun
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  • (College of Chemistry & Bioengineering, Guangxi Colleges and Universities, Key Laboratory of Food Safety and Detection, Guilin University of Technology, Guilin 541004, China)

Received date: 2020-09-01

  Revised date: 2020-09-13

  Online published: 2021-02-03

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Abstract

In order to obtain new microbial strains that can efficiently transform polydatin into resveratrol, four endophytic fungi, numbered as HZ001, HZ002, HZ003 and HZ004 were isolated from Polygonum cuspidatum by selective media using polydatin as the sole carbon source. The activity of four strains of cells and fermentation broth to transform polydatin were identified, and the morphological and molecular biological identification of the strains were carried out. The results of cell transformation assays showed that, polydatin could be transformed into resveratrol by cells of HZ001 and HZ004 with the molar conversion rates of 100%, while no resveratrol was detected in HZ002 and HZ003 cell transformation systems. The results of fermentation broth biotransformation assays showed that polydatin could be transformed into resveratrol by fermentation broths of HZ001, HZ003 and HZ004 with the conversion rates of 55%, 40% and 38%, respectively. The results indicated that HZ001 and HZ004 had the strongest activities to convert polydatin into resveratrol, which mainly depended on the intracellular enzymes. The results of morphological and molecular biological identification indicated that HZ001 and HZ002 were attributed to Aspergillus aculeatus and Penicillium georgiense respectively, both HZ003 and HZ004 were attributed to Aspergillus flavus.

Key words： Polygonum cuspidatum; endophytic fungi; polydatin; resveratrol; biotransformation

Cite this article

YU Jie , XU Qinqian , LI Ziyuan , LIU Hongyan , HAO Zaibin , LI Haiyun . Isolation and identification of endophytic fungi from Polygonum cuspidatum for polydatin transformation[J]. Food and Fermentation Industries, 2021 , 47(1) : 21 -26 . DOI: 10.13995/j.cnki.11-1802/ts.025545

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