To study the inhibitory effect of ClO2 on Alternaria alternata,and provide basis for reducing the loss caused by A.alternata rot during grape storage,isolated bacteria were treated under different concentrations of ClO2 (1.5,3.0,4.5,6.0,7.5,9.0 μg/L) for 10,20 and 30 minutes respectively,the bacteria without any treatment was used as control group.The inhibition effects of ClO2 on A.alternata were explored by measuring of mycelial growth,spore formation,germ tube elongation and observing of the hypha morphology by scanning electron microscopy.The grapes inoculated with A.alternata were treated with 3.0,6.0 and 9.0 μg/L ClO2 respectively for 30 min and stored in the incubator at 28 ℃.The inhibition effect of ClO2 on A.alternata rot was observed by measuring the diameter of lesions,incidence and related enzyme activities.The results showed that ClO2 at different concentrations could significantly inhibit the mycelial growth,spore germination rate and germ tube elongation of Alternaria spp. In vivo experiments inoculated with A.alternata showed that ClO2 treatment could inhibit the diameter of lesions and the rot rate of fruits,delay the accumulation of malondialdehyde content in fruits and maintain high antioxidant activity.Among them,the grapes treated with 9.0 μg/L ClO2 for 30 minutes had the best fresh-keeping effect.
KANG Huifang
,
QIAO Yongjin
,
LIU Chenxia
,
ZHANG Yi
,
Sun Dapeng
,
TONG Xiaoyang
. Inhibitory effect of chlorine dioxide gas on Alternaria alternata[J]. Food and Fermentation Industries, 2021
, 47(2)
: 95
-101
.
DOI: 10.13995/j.cnki.11-1802/ts.023594
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