Advances in proteomics and its application in milk and dairy products

  • JU Ning ,
  • GOU Meng ,
  • ZHANG Tongtong
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  • College of Agriculture, Ningxia University, Yinchuan 750021, China

Received date: 2020-03-22

  Revised date: 2020-08-31

  Online published: 2021-03-08

Abstract

Proteomics technology is an important tool for life science research. It has unique advantages in the fields of medicine, animal and plant research. Proteomics technology can further clarify the expression and their dynamic changes of proteins in milk and dairy products and which has become a research hotspot currently. This paper summarizes the concept, common methods and application fields of proteomics. The application of proteomics in the field of milk and dairy products are introduced emphatically, especially in milk fat globule membrane proteins, whey proteins, milk and dairy product processing, and cheese. And the current problems and limitations are also been discussed. The prospect of proteomics, as well as its application in milk and dairy products, are forecasted which provide a theoretical basis for the in-depth study of milk and dairy products by proteomics technology.

Key words: proteomics; milk; dairy; cheese

Cite this article

JU Ning , GOU Meng , ZHANG Tongtong . Advances in proteomics and its application in milk and dairy products[J]. Food and Fermentation Industries, 2021 , 47(3) : 245 -251 . DOI: 10.13995/j.cnki.11-1802/ts.024032

References

[1] 龙娟,杨晓红,于桂宝.微生物蛋白组学的发展及前景[J].生物技术通报,2006(S1):91-94;99.
LONG J,YANG X H,YU G B.Development and prospect of microbial proteomics[J].Biotechnology Bulletin,2006(S1):91-94;99.
[2] 王延丽, 杨震.蛋白组学及其在食品科学研究中的应用[J].赤峰学院学报(自然科学版),2017,33(17):12-13.
WANG Y L,YANG Z.Proteomics and its application in food science research[J].Journal of Chifeng University(Natural Science Edition),2017,33(17):12-13.
[3] 田尉婧, 张九凯,程海燕,等.基于质谱的蛋白质组学技术在食品真伪鉴别及品质识别方面的应用[J].色谱,2018,36(7):588-598.
TIAN W J,ZHANG J K,CHENG H Y,et al.Applications of mass spectrometry-based proteomics in food authentication and quality identification[J].Chinese Journal of Chromatography,2018,36(7):588-598.
[4] CARVALHO V P,GRASSI M L,PALMA C S,et al.The contribution and perspectives of proteomics to uncover ovarian cancer tumor markers[J].Translational Research,2019,206:71-90.
[5] 陈曼, 何开武,任晓虎,等.帕金森病患者血浆蛋白质组学的初步分析及鉴定[J].新乡医学院学报,2018,35(5):378-381.
CHEN M,HE K W,REN X H,et al.Analysis and identification of plasma proteomics in patients with Parkinson's disease[J].Journal of Xinxiang Medical University,2018,35(5):378-381.
[6] 杨玉英, 刘鹏,贺军,等.雏鸡冷应激前后血清中差异蛋白质组学研究[J].动物营养学报,2017,29(5):1 567-1 573.
YANG Y Y,LIU P,HE J,et al.A study on differential proteomics of chicks serum before and after cold stress[J].Chinese Journal of Animal Nutrition,2017,29(5):1 567-1 573.
[7] SHI X F,WANG X B,CHENG F,et al.iTRAQ-based quantitative proteomics analysis of cold stress-induced mechanisms in grafted watermelon seedlings[J].Journal of Proteomics,2019,192:311-320.
[8] 谢慧, 王风清,魏东芝.蛋白组学差异揭示黑曲霉(Aspergillus niger)高产柠檬酸的分子机制[J].中国酿造,2018,37(7):138-144.
XIE H,WANG F Q,WEI D Z.Molecular mechanism revealed by proteomic differences of Aspergillus niger with high yield citric acid[J].China Brewing,2018,37(7):138-144.
[9] RAPPSILBER J,MANN M.What does it mean to identify a protein in proteomics?[J].Trends in Biochemical Sciences,2002,27(2):74-78.
[10] 庄桂龙, 王金鹏,孙艳,等.蛋白质组学在乳蛋白研究中的应用进展[J].家畜生态学报,2017,38(3):1-4;74.
ZHUANG G L,WANG J P,SUN Y,et al.Application of proteomics in milk protein research[J].Journal of Domestic Animal Ecology,2017,38(3):1-4;74.
[11] 李莲, 唐娟,吴洁,等.基于iTRAQ技术的脂多糖刺激下奶牛乳腺上皮细胞的蛋白组学分析[J].畜牧兽医学报,2016,47(9):1 853-1 860.
LI L,TANG J,WU J,et al.Analysis of expression protein profiles of bovine mammary epithelial cell based on isobaric tag for relative and absolute quantification proteomics and bioinformatics technique[J].Chinese Journal of Animal and Veterinary Sciences,2016,47(9):1 853-1 860.
[12] 祁忠达, 韦艳,龙水庭,等.基于TMT质谱分析技术筛选水稻根尖响应汞胁迫差异表达蛋白[J].生态学杂志,2019,38(6):1 792-1 799.
QI Z D,WEI Y,LONG S T,et al.Screening differentially expressed proteins in response to mercury stress in rice roots by proteomic quantification based on stable isotope labeling and parallel reaction monitoring[J].Chinese Journal of Ecology,2019,38(6):1 792-1 799.
[13] 乌日娜, 薛亚婷,张平,等.豆酱微生物宏蛋白质组提取及分析[J].食品科学,2017,38(14):17-23.
WU R N,XUE Y T,ZHANG P,et al.Metaproteomic analysis of traditional fermented soybean paste in northeast China[J].Food Science,2017,38(14):17-23.
[14] 胡少达, 郭锦,邹少兰,等.宏蛋白质组学在活性污泥研究中的应用[J].应用与环境生物学报,2016,22(4):725-731.
HU S D,GUO J,ZOU S L,et al.Application of metaproteomics in study of activated sludge[J].Chinese Journal of Applied and Environmental Biology,2016,22(4):725-731.
[15] 张秀红, 张武斌,段江燕.宏蛋白质组学方法对清香大曲蛋白分析[J].食品科技,2015,40(9):258-264.
ZHANG X H,ZHANG W B,DUAN J Y.Identifi cation of crude proteins from Daqu (yeast for making hand liquor) for light-fragrant Chinese liquor by proteomics method[J].Food Science and Technology,2015,40(9):258-264.
[16] 乌日娜, 王茜茜,唐筱扬,等.植物乳杆菌 L.plantarum FS5-5在盐胁迫下的蛋白质组学[J].食品与生物技术学报,2016,35(2):123-128.
WU R N,WANG Q Q,TANG X Y,et al.Proteomics research on salt response of L.plantarum FS5-5 grown at different NaCl concentrations using two-dimensional gel electrophoresis[J].Journal of Food Science and Biotechnology,2016,35(2):123-128.
[17] MANSO MA,LÉONIL J,JAN G,et al.Application of proteomics to the characterisation of milk and dairy products[J].International Dairy Journal,2005,15(6-9):845-855.
[18] 彭磊, 宾捷,卢吉平.蛋白质组学技术在人骨肉瘤血清标志物的应用研究[J].湖南师范大学学报(医学版),2018,15(1):42-46.
PENG L,BIN J,LU J P.Application study on proteomics screening of human osteosarcoma serum biomarkers[J].Journal of Hunan Normal University(Medical Sciences),2018,15(1):42-46.
[19] JI X X,LI X S,MA Y,et al.Differences in proteomic profiles of milk fat globule membrane in yak and cow milk[J].Food Chemistry,2017,221:1 822-1 827.
[20] 齐云霞, 赵小伟,黄冬维,等.超速离心和酸沉淀制备牛乳清的蛋白质组学研究[J].中国畜牧兽医,2019,46(8):2 203-2 210.
QI Y X,ZHAO X W,HUANG D W,et al.Proteomics study on preparation of bovine whey by ultracentrifugation and acid precipitation[J].China Animal Husbandry & Veterinary Medicine,2019,46(8):2 203-2 210.
[21] JARDIN J,MOLLE D,PIOT M,et al.Quantitative proteomic analysis of bacterial enzymes released in cheese during ripening[J].International Journal of Food Microbiology,2012,155(1-2):19-28.
[22] 刘小莉, 董明盛.蛋白质组学在乳及乳制品特性研究中的应用[J].中国乳品工业,2005,33(11):34-37.
LIU X L,DONG M S.Application of proteomics to the characterisation of milk and dairy products[J].China Dairy Industry,2005,33(11):34-37.
[23] 靳登鹏, 周雄,刘焕,等.基于免疫亲和层析和ProteoMiner处理的母乳乳清蛋白质组学分析[J].食品科学,2020,41(24):54-60.
JIN D P,ZHOU X,LIU H,et al.Proteomics analysis of breast milk whey based on immunoaffinity chromatography and ProteoMiner[J].Food Science,2020,41(24):54-60.
[24] 张志燕, 马海乐,杨艳华.蛋白质组学及其在醋酸菌研究中的应用[J].食品科学,2016,37(5):259-264.
ZHANG Z Y,MA H L,YANG Y H.Advances in proteomics and its application in acetic acid bacteria research[J].Food Science,2016,37(5):259-264.
[25] 刘伟, 刘书广,韩留福.蛋白质组学及其研究技术概述[J].生物学教学,2018,43(5):4-6.
LIU W,LIU S G,HAN L F.A brief review of proteomics and its research technique[J].Biology Teaching,2018,43(5):4-6.
[26] 芦晶, 刘鹭,张书文,等.蛋白质组学技术在乳品研究中的应用[J].生物技术进展,2013,3(6):385-388.
LU J,LIU L,ZHANG S W,et al.Application of proteomics in bovine milk research[J].Current Biotechnology,2013,3(6):385-388.
[27] 谢秀枝, 王欣,刘丽华,等.iTRAQ技术及其在蛋白质组学中的应用[J].中国生物化学与分子生物学报,2011,27(7):616-621.
XIE X Z,WANG X,LIU L H,et al.iTRAQ technology and its application in proteomics[J].Chinese Journal of Biochemistry and Molecular Biology,2011,27(7):616-621.
[28] CHEN D,LI X Y,ZHAO X,et al.Proteomics and microstructure profiling of goat milk protein after homogenization[J].Journal of Dairy Science,2019,102(5):3 839-3 850.
[29] 杨梅, 武俊瑞,彭秀明,等.牛乳中不同部分蛋白质的组成及功能分析[J].现代食品科技,2018,34(4):11-17;68.
YANG M,WU J R,PENG X M,et al.Analysis of the composition and function of different part protein in milk[J].Modern Food Science and Technology,2018,34(4):11-17;68.
[30] ANAGNOSTOPOULOS A K,KATSAFADOU A I,PIERROS V,et al.Milk of Greek sheep and goat breeds;characterization by means of proteomics[J].Journal of Proteomics,2016,147:76-84.
[31] PETRELLA G,PATI S,GAGLIARDI R,et al.Study of proteolysis in river buffalo mozzarella cheese using a proteomics approach[J].Journal of Dairy Science,2015,98(11):7 560-72.
[32] 陈念念, 方真,陈佳娴,等.基于质谱技术的蛋白质组学在水产品鉴伪中的研究进展[J].食品安全质量检测学报,2018,9(21):5 629-5 636.
CHEN N N,FANG Z,CHEN J X,et al.Research progress of application of proteomics in the aquatic products authentication based on mass spectrometry[J].Journal of Food Safety & Quality,2018,9(21):5 629-5 636.
[33] CHEN D,LI X,ZHAO X,et al.Comparative proteomics of goat milk during heated processing[J].Food Chemistry,2019,275:504-514.
[34] BECK K L,WEBER D,PHINNEY B S,et al.Comparative pproteomics of human and macaque milk reveals species-specific nutrition during postnatal development[J].Journal of Proteome Research,2015,14(5):2 143-2 157.
[35] 杨梅, 徐鑫,张居明,等.蛋白质组学在人乳及牛乳蛋白研究中的应用现状[J].食品与发酵工业,2014,40(11):182-187.
YANG M,XU X,ZHANG J M,et al.The advance of proteomics research applications in human milk and bovine milk[J].Food and Fermentation Industries,2014,40(11):182-187.
[36] 姬晓曦, 马莺.水牛和奶牛乳脂肪球膜蛋白组成和功能性的比较[J].哈尔滨工业大学学报,2017,49(8):104-108.
JI X X,MA Y.Comparison of the protein compositions and functional properties for bovine and buffalo MFGMPs[J].Journal of Harbin Institute of Technology,2017,49(8):104-108.
[37] 杨梅, 彭秀明,武俊瑞,等.人乳与牛乳乳脂肪球膜蛋白质组的对比研究[J].现代食品科技,2016,32(8):284-289;89.
YANG M,PENG X M,WU J R,et al.Comparative study on milk fat globule membrane proteomes in human and bovine milk[J].Modern Food Science and Technology,2016,32(8):284-289;89.
[38] LU J,LIU L,PANG X,et al.Comparative proteomics of milk fat globule membrane in goat colostrum and mature milk[J].Food Chemistry,2016,209:10-6.
[39] YANG Y X,ZHENG N,ZHAO X,et al.Proteomic characterization and comparison of mammalian milk fat globule proteomes by iTRAQ analysis[J].Journal of Proteomics,2015,116:34-43.
[40] 赵小伟, 杨永新,黄冬维,等.牛奶和山羊奶中乳脂球膜蛋白的比较研究[J].中国畜牧兽医,2016,43(11):2 963-2 969.
ZHAO X W,YANG Y X,HUANG D W.Comparison of milk fat globule membrane proteins in milk samples of dairy cow and goat[J].China Animal Husbandry & Veterinary Medicine,2016,43(11):2 963-2 969.
[41] SUN Y X,WANG C N,SUN X M,et al.Comparative proteomics of whey and milk fat globule membrane proteins of Guanzhong goat and Holstein cow mature milk[J].Journal of Food Science,2019,84(2):244-253.
[42] MEDEIROS G,QUEIROGA R,COSTA W K A,et al.Proteomic of goat milk whey and its bacteriostatic and antitumour potential[J].International Journal of Biological Macromolecules,2018,113:116-123.
[43] 古丽巴哈尔·卡吾力,李玲,常占瑛,等.鲜马奶乳清蛋白质组学研究[J].中国乳品工业,2018,46(9):13-18.
GULIBAHAR K,LI L,CHANG Z Y,et al.Study on the protein components of whey protein in fresh mare's milk[J].China Dairy Industry,2018,46(9):13-18.
[44] 张新浩, 李海静,刘景辉,等.德州驴乳清蛋白差异蛋白质组学研究[J].中国草食动物科学,2019,39(2):1-4;8.
ZHANG X H,LI H J,LIU J H,et al.Study of differential proteomics of Dezhou donkey whey proteins[J].China Herbivore Science,2019,39(2):1-4;8.
[45] THANISLASS J S G,SIVAPRAKASAM P,ANTONY P X,et al.Immuno-detection of C3a,a C3 complement activated product in mastitis milk,a potential diagnostic marker[J].Veterinary Sciences,2017,4(1):13-21.
[46] 臧长江,王加启,杨永新,等.热处理牛乳中乳蛋白变化的比较蛋白质组学的研究[J].畜牧兽医学报,2012,43(11):1 754-1 759.
ZANG C J,WANG J Q,YANG Y X,et al.Comparative proteomics analysis of changes of the milk protein during thermal treatment[J].Chinese Journal of Animal and Veterinary Sciences,2012,43(11):1 754-1 759.
[47] 刘翠, 芦晶,张维,等.山羊乳蛋白质及其组学分析[J].中国食品学报,2019,19(5):295-301.
LIU C,LU J,ZHANG W,et al.Analysis of the protein and proteomics of goat milk[J].Journal of Chinese Institute of Food Science and Technology,2019,19(5):295-301.
[48] 刘鸣畅, 吴亚君,王斌,等.采用双向电泳技术研究牛乳热加工后蛋白质组变化[J].中国乳品工业,2016,44(3):23-26;45.
LIU M C,WU Y J,WANG B,et al.Proteomics change of heat-treated milk by two-dimensional electrophoresis technology[J].China Dairy Industry,2016,44(3):23-26;45.
[49] FELFOUL I,JARDIN J,GAUCHERON F,et al.Proteomic profiling of camel and cow milk proteins under heat treatment[J].Food Chemistry,2017,216:161-9.
[50] 罗洁, 任发政,王紫薇,等.干酪质构与风味控制技术研究进展[J].农业机械学报,2016,47(1):190-201;208.
LUO J,REN F Z,WANG Z W,et al.Advances in control technology of cheese quality and texture[J].Transactions of the Chinese Society for Agricultural Machinery,2016,47(1):190-201;208.
[51] PAPPA E C,ROBERTSON J A,RIGBY N M,et al.Application of proteomic techniques to protein and peptide profiling of Teleme cheese made from different types of milk[J].International Dairy Journal,2008,18(6):605-614.
[52] IZQUIERDO-GONZALEZ J J,AMIL-RUIZ F,ZAZZU S,et al.Proteomic analysis of goat milk kefir:Profiling the fermentation-time dependent protein digestion and identification of potential peptides with biological activity[J].Food Chemistry,2019,295:456-465.
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