Food and Fermentation Industries >

2021 , Vol. 47 >Issue 6: 208 - 213

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.025220

Identification and isolation of Staphylococcus aureus in ready-to-eat food imported from Yunnan border trade

  • YAN Mingxing ,
  • CHEN Yun ,
  • LYU Tingbao ,
  • GUAN Dingru ,
  • LI Duhui ,
  • HE Junrong ,
  • LIANG Wei ,
  • YANG Lingchun ,
  • HU Yongjin ,
  • ZHU Renjun
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  • 1(College of Food Science and Technology, Yunnan Agricultural University, Kunming 650000, China)
    2(Kunming Customs Technical Center, Kunming 650000, China)
    3(Kunming Customs, Kunming 650000, China)

Received date: 2020-07-30

  Revised date: 2020-09-14

  Online published: 2021-04-15

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Abstract

In order to investigate the contamination of Staphylococcus aureus in the imported ready-to-eat foods from border trade in Yunnan province, 459 samples of 10 categories collected from 5 border port cities were examined for S. aureus. Biochemical identification, automatic microbial identification (VITEK) and 16S rDNA sequence identification were performed for the isolation of suspicious S. aureus. At the same time, the phylogenetic tree and homology analysis were constructed by using 16S rDNA sequence. The results showed that the detection rate of S. aureus was 0.43%, all of which were candy and chocolate, accounting for 3.8% of this category. Nine strains of S. aureus were isolated from two positive foods, according to phylogenetic analysis of 16S rDNA phylogenetic tree and genetic evolution, these S. aureus strains did not show significant genetic and evolutionary differences in different sample sources. The results provide theoretical and technical support for the establishment of foodborne pathogenic bacteria tracing technology and pathogenic bacteria safety-warning platform for border trade food import in Yunnan province.

Key words： ready-to-eat food; imported food; Staphylococcus aureus; 16S rDNA; bacteria identification

Cite this article

YAN Mingxing , CHEN Yun , LYU Tingbao , GUAN Dingru , LI Duhui , HE Junrong , LIANG Wei , YANG Lingchun , HU Yongjin , ZHU Renjun . Identification and isolation of Staphylococcus aureus in ready-to-eat food imported from Yunnan border trade[J]. Food and Fermentation Industries, 2021 , 47(6) : 208 -213 . DOI: 10.13995/j.cnki.11-1802/ts.025220

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