Food and Fermentation Industries >

2021 , Vol. 47 >Issue 7: 8 - 13

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.025310

High-throughput screening of Streptomyces actuosus for high production of nosiheptide

  • ZHANG Xiaopeng ,
  • CHEN Guicai ,
  • YAN Fajie ,
  • GUO Likun ,
  • ZENG Weizhu ,
  • ZHOU Jingwen
Expand
  • 1(Gansu Huineng Biological Engineering Co.Ltd.,Wuwei 733000,China)
    2(Zhejiang Huineng Biological Engineering Co.Ltd.,Haining 314422,China)
    3(Key Laboratory of Industrial Biotechnology of Ministry of Education,School of Biotechnology,Jiangnan University,Wuxi 214122,China)

Received date: 2020-08-08

  Revised date: 2020-09-08

  Online published: 2021-05-20

Fold

Abstract

In order to enhance the production of nosiheptide by Streptomyces actuosus, the atmospheric and room temperature plasma (ARTP) mutagenesis was carried out on an industrial strain 11-27, and then the obtained mutant was applied in industrial production. Firstly, a high throughput screening method was established according to the molecular characteristics of nosiheptide, which could form complex chromogenic reagent with FeCl3. Arter the treatment of ARTP on strain 11-27, a high-production mutant 22-3B6 was obtained, its production of nosiheptide was increased by 13.7%. Genetic stability analysis showed that the high-titer strain had a stable performance in nosiheptide production. Sixteen batches of fermentation of strain 22-3B6 in a 50 m3 fermenter showed that the average production of nosiheptide was 10.1% higher than that of the original strain 11-27. The high-throughput screening method established here could obtain excellent performance mutant simply and quickly, thus effectively reducing the industrial cost of nassipeptide production.

Key words： nosiheptide; Streptomyces actuosus; high-throughput screening; atmospheric and room temperature plasma(ARTP); mutagenesis

Cite this article

ZHANG Xiaopeng , CHEN Guicai , YAN Fajie , GUO Likun , ZENG Weizhu , ZHOU Jingwen . High-throughput screening of Streptomyces actuosus for high production of nosiheptide[J]. Food and Fermentation Industries, 2021 , 47(7) : 8 -13 . DOI: 10.13995/j.cnki.11-1802/ts.025310

References

[1] HASTE N M,THIENPHRAPA W,TRAN D N,et al.Activity of the thiopeptide antibiotic nosiheptide against contemporary strains of methicillin-resistant Staphylococcus aureus[J].The Journal of Antibiotics,2012,65(12):593-598.
[2] BAI X B,GUO H,CHEN D D,et al.Isolation and structure determination of two new nosiheptide-type compounds provide insights into the function of the cytochrome P450 oxygenase NocV in nocathiacin biosynthesis[J].Organic Chemistry Frontiers,2020,7(3):584-589.
[3] 史莹华, 张伟毅,王先科,等.那西肽对肉仔鸡生长性能及胴体品质的影响.[J].中国抗生素杂志,2010,35(2):152-155.
SHI Y,ZHANG W,WANG X,et al.The effects of nosiheptide on growth performance and carcass quality of broilers[J].Chinese Journal of Antibiotics,2010,35(2):152-155.
[4] WOJTAS K P,RIEDRICH M,LU J Y,et al.Total synthesis of nosiheptide[J].Angewandte Chemie International Edition,2016,55(33):9 772-9 776.
[5] WANG S,ZHOU S,LIU W.Opportunities and challenges from current investigations into the biosynthetic logic of nosiheptide-represented thiopeptide antibiotics[J].Current Opinion in Chemical Biology,2013,17(4):626-634.
[6] WANG B,LAMATTINA J W,MARSHALL S L,et al.Capturing intermediates in the reaction catalyzed by NosN,a class c radical S-adenosylmethionine methylase involved in the biosynthesis of the nosiheptide side-ring system[J].Journal of the American Chemical Society,2019,141(14):5 788-5 797.
[7] DING W,JI W,WU Y,et al.Biosynthesis of the nosiheptide indole side ring centers on a cryptic carrier protein NosJ[J].Nature Communications,2017,8(1):437.
[8] JIN L,WU X,XUE Y,et al.Mutagenesis of NosM leader peptide reveals important elements in nosiheptide biosynthesis[J].Applied Microbiology and Biotechnology,2017,83(4).DOI:10.1128/AEM.02880-16.
[9] WU X,JIN L,ZHANG H,et al.Identification of truncated form of NosP as a transcription factor to regulate the biosynthesis of nosiheptide[J].FASEB Journal,2018,32(1):453-465.
[10] 薛正莲,秦艳飞,王洲,等.激光复合诱变选育那西肽高产菌及其代谢特性的研究[J].食品与发酵工业,2011,37(8):122-125.
XUE Z,QIN Y,WANG Z,et al.Breeding of high-yield nosiheptide strain by laser compound mutation and studying on metabolic properties[J].Food and Fermentation Industries,2011,37(8):122-125.
[11] 公维亮,薛正莲,周扬,等.原生质体诱变及融合选育那西肽高产菌株[J].中国抗生素杂志,2016,41(5):335-339;381.
GONG W,XUE Z,ZHOU Y,et al.Screening of high nosiheptide-producing strain by protoplast mutation and fusion[J].Chinese Journal of Antibiotics,2016,41(5):335-339;381.
[12] YANG Q,GAO H,ZHANG W,et al.Simultaneous hybrid modeling of a nosiheptide fermentation process using particle swarm optimization[J].Chinese Journal of Chemical Engineering,2016,24(11):1 631-1 639.
[13] ZHANG X,ZHANG X F,LI H P,et al.Atmospheric and room temperature plasma (ARTP) as a new powerful mutagenesis tool[J].Applied Microbiology and Biotechnology,2014,98(12):5 387-5 396.
[14] 李英英,杭海峰,庄英萍,等.常压常温等离子体(ARTP)诱变选育顶头孢霉高产菌[J].中国抗生素杂志,2017,42(11):945-950.
LI Y,HANG H,ZHUANG Y,et al.High throughput screening of high-yield cephalosporin C producing strain by mutagenesis using atmospheric and room temperature plasma (ARTP)[J].Chinese Journal of Antibiotics,2017,42(11):945-950.
[15] CAO X,LUO Z,ZENG W,et al.Enhanced avermectin production by Streptomyces avermitilis ATCC 31267 using high-throughput screening aided by fluorescence-activated cell sorting[J].Applied Microbiology and Biotechnology,2018,102(2):703-712.
[16] ZHANG K,MOHSIN A,DAI Y,et al.Combinatorial effect of ARTP mutagenesis and ribosome engineering on an industrial strain of Streptomyces albus S12 for enhanced biosynthesis of salinomycin[J].Frontiers in Bioengineering and Biotechnology,2019,7:212.
[17] ZENG W,DU G,CHEN J,et al.A high-throughput screening procedure for enhancing alpha-ketoglutaric acid production in Yarrowia lipolytica by random mutagenesis[J].Process Biochemistry,2015,50(10):1 516-1 522.
[18] MA F,CHUNG M T,YAO Y,et al.Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform[J].Nature Communications,2018,9:1030.
[19] LEAVELL M D,SINGH A H,KAUFMANN-MALAGA B B.High-throughput screening for improved microbial cell factories,perspective and promise[J].Current Opinion in Biotechnology,2020,62:22-28.
[20] ZENG W,GUO L,XU S,et al.High-throughput screening technology in industrial biotechnology[J].Trends in Biotechnology,2020,38(8):888-906.
[21] LU Y,WANG L,MA K,et al.Characteristics of hydrogen production of an Enterobacter aerogenes mutant generated by a new atmospheric and room temperature plasma (ARTP)[J].Biochemical Engineering Journal,2011,55(1):17-22.
[22] LUO Z,ZENG W,DU G,et al.A high-throughput screening procedure for enhancing pyruvate production in Candida glabrata by random mutagenesis[J].Bioprocess and Biosystems Engineering,2017,40(5):693-701.
[23] XIE J,SONG X,ZHANG Y,et al.Rapid determination of nosiheptide in feed based on dispersive SPE coupled with HPLC[J].Journal of Separation Science,2019,42(3):706-715.
[24] CHEN C Y,YU C,CHEN S W,et al.Effect of yeast with bacteriocin from rumen bacteria on growth performance,caecal flora,caecal fermentation and immunity function of broiler chicks[J].Journal of Agricultural Science,2013,151(2):287-297.
[25] HAO H,CHENG G,IQBAL Z,et al.Benefits and risks of antimicrobial use in food-producing animals[J].Frontiers in Microbiology,2014,5:288.
[26] WANG Q,ZHANG D,LI Y,et al.Genome shuffling and ribosome engineering of Streptomyces actuosus for high-yield nosiheptide production[J].Applied Biochemistry and Biotechnology,2014,173(6):1 553-1 563.
[27] 周扬,薛正莲,夏俊,等.常压室温等离子体(ARTP)诱变及高通量筛选那西肽高产菌株[J].工业微生物,2015,45(2):7-12.
ZHOU Y,XUE Z,XIA J,et al.Mutation by using atmospheric and room temperature plasmas and high-throughput screening method for improving production of nosiheptide of Streptomyces actuosu[J].Industrial Microbiology,2015,45(2):7-12.
[28] 程伟鸽,桂春,鞠建华,等.1株海洋来源Streptomyces sp.SCSIO 1682中那西肽(nosiheptide)的分离鉴定及其生物合成基因簇分析[J].中国海洋药物,2016,35(6):7-14.
CHEN W,GUI C,JU J,et al.Isolation of nosiheptide and identification of its biosynthetic gene cluster from the marine sediment derived Streptomyces sp.SCSIO 1682[J].Chinese Journal of Marine Drug,2016,35(6):7-14.
[29] LI J,LI Y,NIU G,et al.NosP-regulated nosiheptide production responds to both peptidyl and small-molecule ligands derived from the precursor peptide[J].Cell Chemical Biology,2018,25(2):143-153.
Options
Outlines

/

〈 〉

Powered by Beijing Magtech Co. Ltd,.