A Pholiota adiposa strain PA-008 with a high β-glucan-producing property (GenBank accession number:MF687867) was obtained by isolation and purification.In order to further improve the production and yield of β-glucan of strain, single-factor and orthogonal experiments were carried out to design and optimize the medium composition and liquid fermentation conditions of PA-008.The results showed that the optimal fermentation conditions of the strains of PA-008 were as follows:glucose, yeast powder, fermentation temperature 25 ℃, initial pH value 6.0.Meanwhile, an in vitro digestion model was used to analyze the bioavailability of the main nutrients (amino acids, minerals, and β-glucans) in the P.adiposa.The results showed that there was a certain degree of amino acids and minerals release in the mycelium of the P.adiposa.And the release of heavy metal minerals (Pb, Cd, As) were lower than the safe value after in vitro simulated digestion.The β-glucose content in the digestive juice was 41.92 mg/g, and its bioavailability reached 15.50%.The above data provided a theoretical basis for a more scientific and in-depth evaluation of the P.adiposa.
HAN Wanyun
,
ZHANG Changyi
,
GU Zepeng
,
DUAN Xiaoyu
,
SUN Qingjie
,
QIU Lizhong
,
BIAN Xiliang
,
WU Yinglong
,
LIU Yuntao
. Isolation,identification and in vitro simulated digestion of Pholiota adiposa with a high production of β-glucan[J]. Food and Fermentation Industries, 2021
, 47(7)
: 51
-57
.
DOI: 10.13995/j.cnki.11-1802/ts.025773
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