Food and Fermentation Industries >

2021 , Vol. 47 >Issue 7: 64 - 70

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026510

Screening and identification of an AFB1-dergrading strain and product analysis

  • TANG Ying ,
  • DENG Zhanrui ,
  • HUANG Jia ,
  • YANG Xiaonan
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  • (Gansu Province Product Quality Supervision and Inspection Research Institute,Lanzhou 730050,China)

Received date: 2020-12-24

  Revised date: 2021-02-02

  Online published: 2021-05-20

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Abstract

Aflatoxin seriously threatens human health and causes serious economic losses and arouses widespread concern in food safety.In order to develop effective biological bacterium agent against aflatoxin contamination,this study used preliminary screened strain WTX1 as the research object,the AFB1 degradation rate was up to 83.5%.Through morphological,physiological and biochemical analysis and the evolution of 16S rRNA system,the bacterial strain was screened as Bacillus subtilis.By UPLC-MS/MS determination of degradation rate of strain component parts,the degradation liquid mass spectrum was analyzed by software.The secondary metabolites produced during the growth process played a major role in the degradation of AFB1.The mechanism of AFB1 degradation by strain WTX1 is to destroy the toxic structure of AFB1,namely,furan ring dihydrogen double bond and the lipid ring structure of vanillin to achieve the detoxification effect.The experiment will further analyze the effects of the degradation products of the strain WTX1 on human and animal metabolism.

Key words： aflatoxin B1; Bacillus subtilis; UPLC-MS/MS; degradation products; mass spectrometry analysis

Cite this article

TANG Ying , DENG Zhanrui , HUANG Jia , YANG Xiaonan . Screening and identification of an AFB1-dergrading strain and product analysis[J]. Food and Fermentation Industries, 2021 , 47(7) : 64 -70 . DOI: 10.13995/j.cnki.11-1802/ts.026510

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