Food and Fermentation Industries >

2021 , Vol. 47 >Issue 10: 116 - 123

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.025831

Cytoprotective effect and safety of Morchella esculenta protein hydrolysate and its selenized derivative

  • ZHANG Qiang ,
  • WANG Mengjin ,
  • MA Yuhan ,
  • SUN Yujun ,
  • WANG Songhua
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  • (College of Life and Health Sciences, Anhui Science and Technology University, Fengyang 233100, China)

Received date: 2020-10-09

  Revised date: 2020-11-09

  Online published: 2021-06-17

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Abstract

The cytoprotective effects and safety of Morchella protein hydrolysate (MPH) and its selenized derivative (Se-MPH) were studied in this paper.The cytoprotective experiment, performed by employing an H2O2-induced Caco-2 cell model, revealed that both MPH and Se-MPH could protect Caco-2 cells from oxidative injury by alleviating oxidative stress and inhibiting apoptosis.This protective effect may be mainly attributed to the activation of the Nrf2 signaling pathway and the regulation of apoptotic-related protein expressions.The safety evaluation, conducted via cytotoxicity analysis, erythrocyte hemolysis assay, Ames test, and CAM assay, demonstrated no cytotoxicity, hemolytic activity, and mutagenic potential for MPH and Se-MPH.They neither caused visible morphological changes in blood vessels nor led to bleeding in the CAM assay.All these findings suggest that MPH and Se-MPH possess remarkable cytoprotective effects and biological safety, and therefore are expected to be developed and utilized as a potential nutraceutical, functional, or pharmaceutical ingredients.

Key words： Morchella esculenta; protein hydrolysate; selenium; oxidative stress; cytoprotective effect; safety

Cite this article

ZHANG Qiang , WANG Mengjin , MA Yuhan , SUN Yujun , WANG Songhua . Cytoprotective effect and safety of Morchella esculenta protein hydrolysate and its selenized derivative[J]. Food and Fermentation Industries, 2021 , 47(10) : 116 -123 . DOI: 10.13995/j.cnki.11-1802/ts.025831

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