Food and Fermentation Industries >

2021 , Vol. 47 >Issue 10: 159 - 163

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.025998

Screening and identification of pyrroloquinoline quinone producing strains

  • ZHU Daoyang ,
  • WEI Chunyu ,
  • YU Xiaobin
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  • (School of Biotechnology, The Key Laboratory of Industrial Biotechnology, Ministry of Education,Jiangnan University, Wuxi 214122, China)

Received date: 2020-10-25

  Revised date: 2020-12-15

  Online published: 2021-06-17

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Abstract

This study established rapid high performance liquid chromatography method for the detection of pyrroloquinoline quinone (PQQ). In order to screen the strains that produce PQQ, and increase the fermentation yield of PQQ, a selective medium with methanol was adopted as the sole carbon source. Spectroscopy was used for preliminary screening of the samples, HPLC was used for re-screening, and the fermentation medium of the selected strains to produce PQQ was optimized. The results showed that 56 PQQ-producing strains were screened from the samples, and the highest yield was 20.6 mg/L. Through 16s rDNA sequencing analysis, the strain was identified as Methylopila sp.. The methanol content in the fermentation medium was also optimized. When the methanol volume concentration was 2.5%, the PQQ yield reached 26.3 mg/L, which was 28% higher than that in the initial medium with 1.0% methanol. The HPLC method established in this study provided a rapid detection method for the screening of PQQ high-yielding strains. The selected strains had high methanol tolerance and PQQ production potential, which could provide a reference for further PQQ fermentation research.

Key words： pyrroloquinoline quinone(PQQ); Methylopila sp.; selective medium; spectroscopy; HPLC

Cite this article

ZHU Daoyang , WEI Chunyu , YU Xiaobin . Screening and identification of pyrroloquinoline quinone producing strains[J]. Food and Fermentation Industries, 2021 , 47(10) : 159 -163 . DOI: 10.13995/j.cnki.11-1802/ts.025998

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