Food and Fermentation Industries >

2021 , Vol. 47 >Issue 10: 203 - 206

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.025904

A fluorescent sensing system based on Genefinder for detection of zearalenone

  • GUO Ting ,
  • CHEN Jinhang ,
  • ZHOU Hongyuan ,
  • ZHANG Yuhao ,
  • MA Liang
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  • (College of Food Science, Southwest University, Chongqing 400715, China)

Received date: 2020-10-20

  Revised date: 2020-12-03

  Online published: 2021-06-17

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Abstract

Zearalenone (ZEN) is a kind of toxin produced by Fusarium fungi. It has reproductive toxicity, immunotoxicity, hepatorenal toxicity and carcinogenicity, and poses a serious threat to human health. In this work, a facile and sensitive fluorescence sensing system was developed for the detection of ZEN. Aptamer as recognition element could recognize the target ZEN. Genefinder was chosen as the sensing signal, the fluorescence signal will increase due to the interaction between Genefinder and double DNA which formed by ZEN aptamer and its complementary strand. But in the presence of ZEN, the aptamer could recognize the ZEN, leading to decrease of fluorescence. The results showed that the sensing system was high selectivity for ZEN, and the detection linear range was 0.1-200 μg/L with 0.1 μg/L the detection limit under the optimized condition. For real corn flour sample, the standard recovery ranges from 100.4% to 105.8%. The fluorescence sensing system has been successfully used for the detection of ZEN in the real sample which showed a wide application potential.

Key words： zearalenone; aptamer; Genefinder; fluorescence sensing system; high sensitive

Cite this article

GUO Ting , CHEN Jinhang , ZHOU Hongyuan , ZHANG Yuhao , MA Liang . A fluorescent sensing system based on Genefinder for detection of zearalenone[J]. Food and Fermentation Industries, 2021 , 47(10) : 203 -206 . DOI: 10.13995/j.cnki.11-1802/ts.025904

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