A high performance liquid chromatographic method was developed for the simultaneous determination of free guanine, hypoxanthine, xanthine and adenine in 15 Huangjiu (Chinese rice wine) samples. To reduce matrix interferences and increase method sensitivity, MCX mixed cation exchange solid phase extraction column was employed for sample clean-up. After the sample was boiled to remove ethanol, it was purified by MCX column and analyzed by high performance liquid chromatography. The standard curve of purines showed good linearity in the range of 0.1-25 mg/L, with the correlation coefficient R larger than 0.999 9. The lowest detectable concentrations of guanine, hypoxanthine, xanthine and adenine were 0.1, 0.1, 0.2, 0.1 mg/L, respectively. The recovery rates were between 83.13%-98.32%. The intra-day and inter-day assay precision (RSD) of these four purines were less than 4.0%. This method is simple, fast and reliable.
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