In this study, several metabolic engineering strategies were used to improve the synthesis of L-histidine in Escherichia coli. Firstly, the precursor region of L-histidine operon in E. coli was replaced by an artificial open reading frame (hisL'-λattB'-trpE), and the titer of L-histidine was increased to 872.50 mg/L after the expression of hisGE271K. Secondly, the enzyme activities was compared under different concentrations of inhibitors, and L-histidine titer was analyzed when 11 kinds of hisG from Corynebacterium glutamicum ATCC 130132, Serratia marcescens ZJZ626 and E. coli BL21 (DE3) were expressed. The optimal hisG was selected and L-histidine titer was increased to 1 480.42 mg/L. Next, CRISPR/Cas9 was used to integrate the optimal hisG in the genome. Finally, after comparing the effects of two different Prs on L-histidine production, the better one was selected and integrated in the genome, resulting in the L-histidine titer of 3 898.06 mg/L in 72 h and 5 574.63 mg/L in 96 h. This work laid a good foundation for the industrial production of L-histidine in the future.
LI Mengying
,
LYU Xueqin
,
LIU Yanfeng
,
LI Jianghua
,
DU Guocheng
,
WU Jianrong
,
LIU Long
. Metabolic engineering of Escherichia coli for increased synthesis of L-histidine[J]. Food and Fermentation Industries, 2021
, 47(12)
: 1
-9
.
DOI: 10.13995/j.cnki.11-1802/ts.026161
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