Food and Fermentation Industries >

2021 , Vol. 47 >Issue 12: 216 - 223

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026480

Metabolomic analysis of non-volatile chemical components in Zanthoxylum schinifolium Sieb.et Zucc. from two origins

  • YANG Qingqing ,
  • WANG Zhirong ,
  • PENG Lin ,
  • CHEN Qiaoli ,
  • WEN Leyan ,
  • GUO Zehang ,
  • KAN Jianquan
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  • 1(College of Food Science, Southwest University, Chongqing 400715, China)
    2(Laboratory of Quality & Safety Risk Assessment for Agro-products on Storage and Preservation (Chongqing), Ministry of Agriculture and Rural Affairs, PRC, Chongqing 400715, China)
    3(Chinese-Hungarian Cooperative Research Centre for Food Science, Chongqing 400715, China)

Received date: 2020-12-16

  Revised date: 2021-01-14

  Online published: 2021-07-22

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Abstract

To explore the differences in non-volatile components of Zanthoxylum schinifolium Sieb.et Zucc. from two origins, a non-targeted metabolomics technology based on ultra performance liquid chromatography-quadrupole-electrostatic field orbital trap high resolution mass spectrometry (UPLC-QE-Orbitrap-MS)was explored, and then principal component analysis and orthogonal partial least square discriminant analysis were used to distinguish between them. It was concluded that 74 different metabolites in the two kinds of Zanthoxylum schinifolium Sieb.et Zucc.. Amino acids were up-regulated in the Jiangjin sample, and organic acids, polyphenol flavonoids and sugars were up-regulated in the Jinyang sample. Among them, 9 metabolites with significant differences were screened out as the chemical markers to distinguish the two kinds of Zanthoxylum schinifolium Sieb.et Zucc., including hydroxy-alpha-sanshool, sucrose, trehalose, raffinose, quinic acid, shikimic acid, 2-isopropylmalic acid, L-arginine and L-lysine. In addition, the possible mechanism for the difference of non-volatile components was further elaborated in order to provide a theoretical basis for the intensive processing of the two Zanthoxylum schinifolium Sieb.et Zucc. and select excellent germplasm resources in the future.

Key words： Zanthoxylum schinifolium Sieb.et Zucc.; untargeted metabolomics; UPLC-QE-Orbitrap-MS; non-volatile components; multivariate statistical analysis; chemical markers

Cite this article

YANG Qingqing , WANG Zhirong , PENG Lin , CHEN Qiaoli , WEN Leyan , GUO Zehang , KAN Jianquan . Metabolomic analysis of non-volatile chemical components in Zanthoxylum schinifolium Sieb.et Zucc. from two origins[J]. Food and Fermentation Industries, 2021 , 47(12) : 216 -223 . DOI: 10.13995/j.cnki.11-1802/ts.026480

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