Food and Fermentation Industries >

2021 , Vol. 47 >Issue 12: 250 - 256

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.025865

Determination of five water-soluble vitamins in honey by ultra performance liquid chromatography-tandem mass spectrometry

  • XUE Xia ,
  • ZHAO Huinan ,
  • WEI Lili ,
  • DING Yi ,
  • SU Shufang ,
  • LU Lanxiang ,
  • WANG Jun ,
  • LIU Yanming ,
  • HU Mei
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  • 1(Shandong Institute for Food and Drug Control,Jinan 250101,China)
    2(Shandong Research Center of Engineering and Technology for Safety Inspection of Food and Drug,Jinan 250101,China)

Received date: 2020-10-12

  Revised date: 2020-11-17

  Online published: 2021-07-22

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Abstract

An ultra performance liquid chromatography coupled with tandem-mass spectrometric (UPLC-MS/MS) method was developed for simultaneous determination of thiamine (B1), riboflavin (B2), pantothenic acid (B5), pyridoxine (B6), and biotin (B7) in honey. The samples were extracted with 20 mmol/L ammonium formate solution, and cleaned-up with a HLB solid phase extraction (SPE) column. The extracts were separated on a HSS T3 column with a gradient elution using methanol and 20 mmol/L ammonium formate as mobile phases. Mass spectral acquisition was performed in the positive ion mode by applying multiple reaction monitoring (MRM) with external standard method. The results showed that five water-soluble vitamins showed good linearities in the range of 5-200 ng/mL with correlation coefficients 0.99. The limits of detection(LODs) and limits of quantification (LOQs) for the five water-soluble vitamins were 20 μg/kg and 50 μg/kg respectively. The recoveries of analytes from blank honey at three levels of 50,250 and 500 μg/kg ranged from 90.2% to 108.8% with relative standard deviations less than 15%. This rapid and sensitive method is suitable for the determination of five water-soluble vitamins in honey.

Key words： honey; water-soluble vitamins; ultra performance liquid chromatography coupled with tandem-mass spectrometry; solid phase extraction

Cite this article

XUE Xia , ZHAO Huinan , WEI Lili , DING Yi , SU Shufang , LU Lanxiang , WANG Jun , LIU Yanming , HU Mei . Determination of five water-soluble vitamins in honey by ultra performance liquid chromatography-tandem mass spectrometry[J]. Food and Fermentation Industries, 2021 , 47(12) : 250 -256 . DOI: 10.13995/j.cnki.11-1802/ts.025865

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