To investigate the protective effect of chitosan oligosaccharides (COS) on the cellular damage of SK-N-SH cells induced by H2O2, H2O2 was used to establish an in vitro oxidative damage model. The cell viability was detected by MTT, and the release of lactate dehydrogenase (LDH) was detected by kit. JC-10 was used to detect changes in cell mitochondrial membrane potential, ATP luminescence assay kit was used to detect intracellular ATP level, and the expression of Bcl-2, Bax, Nrf2 and HO-1 protein was detected by Western Blot. Results showed that compared with the H2O2 group, COS pretreatment could effectively increase the cell survival rate induced by H2O2, markedly inhibit the release of intracellular LDH, significantly restore mitochondrial membrane potential, increase intracellular ATP levels, and promote the expression of Bcl-2, Nrf2 and HO-1 protein, inhibit the expression of Bax protein. COS could protect SK-N-SH cells from oxidative damage induced by H2O2 via inhibiting cell apoptosis and promoting the expression of antioxidant proteins.
ZHANG Xiaoxia
,
LI Xiaoxiao
,
SUN Yaxuan
,
DAI Xueling
. Mechanism of chitosan oligosaccharides attenuated oxidative damage induced by H2O2 in SK-N-SH cells[J]. Food and Fermentation Industries, 2021
, 47(14)
: 57
-62
.
DOI: 10.13995/j.cnki.11-1802/ts.027428
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