In order to explore the metabolic pathways of Weissella 10d-17 and preliminarily understand its gene functions, transcriptogram sequencing and bioinformatics correlation analysis of 10d-17 bacteria based on RNA-Seq technology were conducted. The results showed that 10.41 GB of data was obtained, and 6 947 Unigene lines were obtained after filtering out the redundancy. Among them, the number of Q20 (Phred value greater than the percentage of 20 bases in the total base) was 98.36%, and the number of high-quality reads was between 9.83 million and 13.59 million. A total of 1 018 significantly differentially expressed genes (DEGs) were obtained, among which 509 genes were up-regulated and 509 were down-regulated in the experimental group. Gene Ontology (GO) functional annotation information was obtained for 772 genes of DEGs, which were mainly concentrated in RNA metabolism process and drug binding. Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathway annotation found that DEGs were mainly concentrated in biochemical metabolism, biosynthesis of secondary metabolites and other related metabolic pathways, among which 13 genes were significantly differentially expressed in glycolysis and pyruvate metabolic pathways.
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