To increase the content of ε-poly-L-lysine(ε-PL) with high degree of polymerization, membrane was screened and the membrane separation process parameters were optimized. The antibacterial properties of the products before and after separation were compared, while the way ε-PL penetrated the membrane was also analyzed. Results showed that the polyethersulfone(PES) membrane with a molecular weight cut-off of 3 000 Da was the best. At 0.25 MPa, pH 6, and 20 g/L feed solution, the content of ε-PL with a degree of polymerization of 25-35 was increased to 91.22%, and the product after filtering was more conducive to inhibit Staphylococcus aureus and Escherichia coli. The membrane was not able to achieve absolute separation of ε-PL, but it increased the proportion of ε-PL with high polymerization degrees and improved the antibacterial performance. The study is of great significance to improve the application value of ε-PL.
ZHAO Xingyu
,
CHEN Xusheng
,
ZHANG Hongjian
,
ZHANG Jianhua
. Increasing the ε-PL content with high degree of polymerization in products using membrane separation[J]. Food and Fermentation Industries, 2021
, 47(14)
: 196
-201
.
DOI: 10.13995/j.cnki.11-1802/ts.026026
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