Food and Fermentation Industries >

2021 , Vol. 47 >Issue 15: 15 - 21

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026252

Breeding of tannase-producing Aspergillus carbonarius using ARTP mutagenesis and fermentation optimization

  • DONG Xianxian ,
  • WU Xiaojiang ,
  • ZHANG Yulong ,
  • WU Xiaodan ,
  • WAN Yin ,
  • LIU Chengmei ,
  • FU Guiming
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  • 1(State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330000,China)
    2(College of Food Science & Technology,Nanchang University,Nanchang 330000,China)
    3(International Institute of Food Innovation,Nanchang University,Nanchang 330000,China)

Received date: 2020-11-26

  Revised date: 2021-01-07

  Online published: 2021-08-23

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Abstract

In order to obtain high yield of tannase, atmospheric room temperature plasma (ARTP) was used to mutagenize Aspergillus carbonarius FCYN212 and fermentation parameters were optimized. The results showed that NCUF M8 with high tannase activity was successfully selected after ARTP mutagenesis, bromophenol blue circle screening and fermentation. The tannase activity of NCUF M8 was 0.212 U/mL, which was 57% higher than that of parent strain (0.135 U/mL). After eight consecutive generations and fermentation tests, the tannase activity levels were kept at 0.208 U/mL, which indicated that it had a good genetic stability. The optimal fermentation conditions were as followed: fermentation time of 4 d, inoculation of 2%, tannic acid of 60 g/L, dissolved oxygen concentration of 45%, and an addition time of inducer at 36 h. The tannase activity of NCUF M8 reached 1.377 U/mL under the optimized conditions, which was 549% significantly higher than original strain of 0.212 U/mL (P<0.05). The results provided a theoretical basis for the mutation breeding of tannase producing strain. Besides, it could reduce the production cost of tannase industrial production.

Key words： tannase; Aspergillus carbonarius; ARTP; optimization of fermentation parameters

Cite this article

DONG Xianxian , WU Xiaojiang , ZHANG Yulong , WU Xiaodan , WAN Yin , LIU Chengmei , FU Guiming . Breeding of tannase-producing Aspergillus carbonarius using ARTP mutagenesis and fermentation optimization[J]. Food and Fermentation Industries, 2021 , 47(15) : 15 -21 . DOI: 10.13995/j.cnki.11-1802/ts.026252

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