Food and Fermentation Industries >

2021 , Vol. 47 >Issue 15: 29 - 35

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026144

Screening,identification and enzyme characteristics of alginate lyase-producing strains from the gut of Monodonta labio

  • LIU Yaotian ,
  • XI Maosheng ,
  • ZHAO Yang ,
  • LI Yuechan ,
  • LUO Xuegang
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  • 1(College of Bioengineering,Tianjin University of Science and Technology,Tianjin 300457,China)
    2(Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education,Tianjin University of Science and Technology,Tianjin 300457,China)

Received date: 2020-11-11

  Revised date: 2021-01-19

  Online published: 2021-08-23

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Abstract

High-yield alginate lyase producing strain ML17 was screened from the intestinal tract of Monodonta labio in the North-South intertidal zone of China by sodium alginate as the sole carbon source. The strain was identified as Vibrio sp. ML17 (CGMCC NO. 20377) by morphological analysis and 16S rRNA. The results of Wagstsuma blood agar plate analysis showed that ML17 was non-hemolytic. Single factor results showed that ML17 optimal conditions as follows: sodium alginate of 5 g/L, tryptone of 5 g/L, NaCl of 30 g/L, pH 7-8 and 30 ℃. Vibrio sp. ML17 reached the growth plateau after 10 h under the optimal conditions, and the highest enzyme production was achieved after 14 h. In addition, the activity of alginate lyase was 26.31 U/mL. The optimal temperature pH was 40 ℃ and 7.5, respectively. It had certain thermal-stability at 40 ℃ and 45 ℃. Under the condition of 5 mmol/L Na+ and Ca2 +, the activity was significantly promoted, while Fe2 +, Cu2 + and Zn2 + inhibited. Further application results showed that Vibrio sp. ML17 could effectively degrade brown algae, and the degradation rates of kelp and sargassum reached 44.0% and 38.9% after 72 h, respectively. Vibrio sp. ML17 could have good application potential in the preparation of alginate oligo-saccharides and the control and removal of alginate in aquaculture.

Key words： Monodonta labio; intestinal tract; Vibrio sp.; sodium alginate; alginate lyase

Cite this article

LIU Yaotian , XI Maosheng , ZHAO Yang , LI Yuechan , LUO Xuegang . Screening,identification and enzyme characteristics of alginate lyase-producing strains from the gut of Monodonta labio[J]. Food and Fermentation Industries, 2021 , 47(15) : 29 -35 . DOI: 10.13995/j.cnki.11-1802/ts.026144

References

[1] 李杰,张睿,张勇,等.藻类养殖产业发展及其碳汇评估研究[J].江西农业,2019(8):124.
LI J,ZHANG R,ZHANG Y,et al.Research on the development of algae culture industry and its carbon sink assessment[J].Jiangxi Agriculture,2019(8):124.
[2] BALDURSDOTTIR S G,KJONIKSEN A L,KARLSEN J,et al.Riboflavin-photosensitized changes in aqueous solutions of alginate.Rheological studies[J].Biomacromolecules,2003,4(2):429-36.
[3] FALKEBORG M,CHEONG L Z,GIANFICO C,et al.Alginate oligosaccharides:Enzymatic preparation and antioxidant property evaluation[J].Food Chem,2014,164:185-94.
[4] YANG Y,MA Z ,YANG G,et al.Alginate oligosaccharide indirectly affects toll-like receptor signaling via the inhibition of micro RNA-29b in aneurysm patients after endovascular aortic repair[J].Drug Design,Development and Therapy,2017(11):2 565-2 579.
[5] WU J,ZHANG M,ZHAGN Y,et al.Anticoagulant and FGF/FGFR signal activating activities of the heparinoid propylene glycol alginate sodium sulfate and its oligosaccharides[J].Carbohydr Polym,2016.136:641-8.
[6] TONDERVIK A,SLETTA H,KLINKENBERG G,et al.Alginate oligosaccharides inhibit fungal cell growth and potentiate the activity of antifungals against Candida and Aspergillus spp[J].PLoS One,2014.9(11):e112 518.
[7] TAMOSAITYTE S,GALLI R,UCKERMANN O,et al.Biochemical monitoring of spinal cord injury by FT-IR spectroscopy—Effects of therapeutic alginate implant in rat models[J].PLoS One,2015,10(11):e0 142 660.
[8] KIHARA M,SAKATA T.Influences of incubation temperature and various saccharides on the production of organic acids and gases by gut microbes of rainbow trout Oncorhynchus mykiss in a micro-scale batch culture[J].Journal of Comparative Physiology B:Biochemical,Systemic,and Environmental Physiology,2001,171(6):441-447.
[9] BOSE S K,HOWLADER P,JIA X,et al.Alginate oligosaccharide postharvest treatment preserve fruit quality and increase storage life via Abscisic acid signaling in strawberry[J].Food Chem,2019,283:665-674.
[10] ZHU B,YIN H,Alginate lyase:Review of major sources and classification,properties,structure-function analysis and applications[J].Bioengineered,2015,6(3):125-31.
[11] WONG T Y,PRESTON L A,SCHILLER N L,ALGINATE LYASE:Review of major sources and enzyme characteristics,structure-function analysis,biological roles,and applications[J].Annu Rev Microbiol,2000,54:289-340.
[12] SUZUKI H,SUZUKI K,INOUE A,et al.A novel oligoalginate lyase from abalone,Haliotis discus hannai,that releases disaccharide from alginate polymer in an exolytic manner[J].Carbohydr Res,2006,341(11):1809-19.
[13] RAHMAN M M,INOUE A,TANAKA H,et al.Isolation and characterization of two alginate lyase isozymes,AkAly28 and AkAly33,from the common sea hare Aplysia kurodai[J].Comp Biochem Physiol B Biochem Mol Biol,2010,157(4):317-25.
[14] KIM D,BAIK K S,HWANG Y S,et al.Vibrio hemicentroti sp.nov.An alginate lyase-producing bacterium,isolated from the gut microflora of sea urchin (Hemicentrotus pulcherrimus)[J].Int J Syst Evol Microbiol,2013,63(Pt 10):3697-703.
[15] 王一农,曾国权,单齿螺Monodonta labio的实验生态与环境分布[J].海洋科学,1994(3):14-16.
WANG Y N,ZENG G Q,Experimental ecology and environmental distribution of Monodonta labio[J].Marine science,1994(3):14-16.
[16] 刘海超,张健,王共明,等.褐藻胶的降解方法及其产物生物活性研究进展[J].食品工业科技,2020,41(13):350-357;363.
LIU H C,ZHANG J,WANG G M,et al.Research progress on the degradation methods of alginate and the biological activity of its products[J].Food Industry Technology,2020,41(13):350-357;363.
[17] ITO M,K WATANABE K,MARUYAMA T,et al.Enrichment of bacteria and alginate lyase genes potentially involved in brown alga degradation in the gut of marine gastropods[J].Sci Rep,2019,9(1):2 129.
[18] 李双,王碧盈,鲍时翔,等.产褐藻胶裂解酶交替单胞菌的发酵优化及alg2951的外源表达[J].基因组学与应用生物学,2020,39(5):2 088-2 095.
LI S,WANG B Y,BAO S X,et al.Fermentation optimization of Alginate lyase-producing Alternomonas and exogenous expression of alg2951[J].Genomics and Applied Biology,2020,39(5):2 088-2 095.
[19] NARSICO J,INOUE A,OKA S,et al.Production of a novel dimeric 4-deoxy-L-erythro-5-hexoseulose uronic acid by a PL-17 exolytic alginate lyase from Hydrogenophaga sp.UMI-18[J].Biochem Biophys Res Commun,2020,525(4):982-988.
[20] 李云涛,汪立平,张孟,等.鲍鱼来源褐藻胶裂解酶菌株的筛选及发酵条件优化[J].大连海洋大学学报,2017,32(5):574-583.
LI Y T,WANG L P,ZHANG M,et al.Screening of alginate lyase strains from abalone and optimization of fermentation conditions[J].Journal of Dalian Ocean University,2017,32(5):574-583.
[21] DONG S,WEI T D ,CHEN X L,et al.Molecular insight into the role of the N-terminal extension in the maturation,substrate recognition,and catalysis of a bacterial alginate lyase from polysaccharide lyase family 18[J].J Biol Chem,2014,289(43):29 558-29 569.
[22] 李学智.蛭弧菌在草鱼主养池塘应用试验[J].河北渔业,2018(8):41-42;52.
LI X Z.Applicationtest of Budovibrium in grass carp main Pond[J].Hebei Fisheries,2018(8):41-42;52.
[23] 姚艳艳,刘心田,常丽荣,等.鲍鱼肠道海带降解菌株的筛选、鉴定及粗酶学性质研究[J].食品与发酵工业,2019,45(9):61-66.
YAO YY,LIU X T,CHANG L R,et al.Screening,identification and crude enzymatic properties of abalone kelp degrading strains[J].Food and Fermentation Industries,2019,45(9):61-66.
[24] 丁淑妍,侯丽艳,于成勇,等.海洋性弧菌致病机制研究进展[J].职业与健康,2019,35(7):984-989.
DING S Y,HOU L Y,YU C Y,et al.Research progress on pathogenic mechanism of Marine vibrio[J].Occupation andhealth,2019,35(7):984-989.
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