The objective of this study was to select proteases for preparation of DPP-IV inhibitory peptide from yak milk casein and optimize the enzymatic process. The hydrolytic proteases were screened by simulated enzymatic digestion of yak milk casein, and then single and multiple enzyme hydrolysis were carried out. The results showed that the best enzyme combination for the preparation of DPP-IV inhibitory peptide was alkaline protease, papain, and bromelain. The optimal enzymatic process for the preparation of DPP-IV inhibitory peptide was 64 ℃, 460 W of ultrasonic power, 82 min of ultrasonic, 0.5 h hydrolysis of alkaline protease, papain and bromelain with 3 000 U/g in turn. DPP-IV inhibition rate and hydrolysis degree of the final hydrolysate were (67.05±0.79)% and (30.57±0.72)%, respectively. The results indicated that the multiple enzyme hydrolysis combined with ultrasonic pretreatment could be applied to the hydrolysis of yak milk casein to obtain the DPP-IV inhibitory peptide with high active.
WANG Lingli
,
LIU Tongjie
,
ZHANG Lanwei
,
GONG Pimin
,
YI Huaxi
. Proteases for preparation of DPP-IV inhibitory peptide from yak milk casein and process optimization[J]. Food and Fermentation Industries, 2021
, 47(15)
: 137
-141
.
DOI: 10.13995/j.cnki.11-1802/ts.026782
[1] ROGLIC G.WHO Global report on diabetes:A summary[J].International Journal of Noncommunicable Diseases,2016,1:3-8.
[2] DAVID H.Diabetes:Concerns about long-term use of GLP-1 analogues[J].Nature Reviews Endocrinology,2016,12(4):186.
[3] TRICCO A C,ANTONY J,KHAN P A,et al.Safety and effectiveness of dipeptidyl peptidase-4 inhibitors versus intermediate-acting insulin or placebo for patients with type 2 diabetes failing two oral antihyperglycaemic agents:A systematic review and network meta-analysis[J].BMJ Open,2014,4(12):1-10.
[4] NONGONIERMA A B,FITZGERALD R J.Features of dipeptidyl peptidase IV (DPP-IV) inhibitory peptides from dietary proteins[J].Journal of Food Biochemistry,2019,43(1):1-11.
[5] 宋佳佳, 白函瑜,王倩,等.牦牛乳酪蛋白酶解产物二肽基肽酶-Ⅳ抑制活性和特性分析[J].乳业科学与技术,2015,38(1):1-4.
SONG J J,BAI H Y,WANG Q,et al.Dipeptidyl peptidase-IV inhibitory activity and physicochemical properties of yak milk casein hydrolysates[J].Dairy Science and Technology,2015,38(1):1-4.
[6] 张颖. 牛、羊乳酪蛋白源DPP-Ⅳ抑制肽的制备、鉴定及抑制机理研究[D].北京:中国农业大学,2016.
ZHANG Y.Enzymatic preparation,identification and inhibition mechanism of DPP-IV inhibitory peptides derived from bovine and caprine milk casein[D].Beijing:China Agricultural University,2016.
[7] KHANDPUR P,GOGATE P R.Effect of novel ultrasound based processing on the nutrition quality of different fruit and vegetable juices[J].Ultrasonics Sonochemistry,2015,27:125-136.
[8] 蓝尉冰, 韩鑫,陈冠余,等.超声波预处理协同酶提取近江牡蛎活性肽研究[J].食品研究与开发,2018,39(8):48-52.
LAN W B,HAN X,CHEN G Y,et al.Research on extraction of bioactive peptides from ostrea rivularis gould by ultrasonic pretreatment combined with enzyme[J].Food Research and Development,2018,39(8):48-52.
[9] 赵换维. 杏鲍菇多肽制备工艺及体外活性研究[D].西安:西北大学,2019.
ZHAO H W.Study on preparation technology and the activity of polypeptide from pleurotus eryngii in vitro[D].Xi’an:Northwest University,2019.
[10] YAN Z,YUAN L,PENGJIE W,et al.Rennet-induced coagulation properties of yak casein micelles:A comparison with cow casein micelles[J].Food Research International,2017,102:25-31.
[11] 侯虎. 鳕鱼免疫活性肽的可控制备及其免疫活性研究[D].青岛:中国海洋大学,2011.
HOU H.The controllable preparation of pollock immunomodulating peptide and its immunity activity[D].Qingdao:Ocean University of China,2011.
[12] 徐荣荣, 张珍,张盛贵,等.超声波辅助酶法水解酪蛋白工艺研究[J].甘肃农业大学学报,2017,52(6):153-158;163.
XU R R,ZHANG Z,ZHANG S G,et al.Optimum on ultrasonic-associated enzymatic hydrolysis of casein[J].Journal of Gansu Agricultural University,2017,52(6):153-158;163.
[13] KARIMI A,AZIZI M H,GAVLIFHI H A.Fractionation of hydrolysate from corn germ protein by ultrafiltration:In vitro antidiabetic and antioxidant activity[J].Food Science & Nutrition,2020,8(5):2 395-2 405.
[14] LI N,ZHONG Q X.Stable casein micelle dispersions at pH 4.5 enabled by propylene glycol alginate following a pH-cycle treatment[J].Carbohydrate Polymers,2020,233:1-13.
[15] MINKIEWICZ P,IWANIAK A,DAREWICZ M.BIOPEP-UWM database of bioactive peptides:Current opportunities[J].International Journal of Molecular Sciences,2019,20(23):5 978.
[16] UDENIGWE C C.Bioinformatics approaches,prospects and challenges of food bioactive peptide research[J].Trends in Food Science & Technology,2014,36(2):137-143.
[17] DING Y,CHEN L,SHI Y G,et al.Emulsifying and emulsion stabilizing properties of soy protein hydrolysates,covalently bonded to polysaccharides:The impact of enzyme choice and the degree of hydrolysis[J].Food Hydrocolloids,2021,113:1-15.
[18] TAVANO O L.Protein hydrolysis using proteases:An important tool for food biotechnology[J].Journal of Molecular Catalysis B:Enzymatic,2013,90:1-11.
[19] 邢琛. 牛乳酪蛋白深度水解配方食品研究[D].武汉:武汉轻工大学,2019.
XING C.Study on bovine casein deep hydrolysis formula[D].Wuhan:Wuhan Polytechnic University,2019.
[20] 李子超, 王丽娜,李昀锴,等.3种乳源酪蛋白粒径及胶束结构的差异性[J].食品科学,2012,33(5):58-61.
LI Z C,WANG L N,LI Y K,et al.Differences in particle size and structure of casein micelle from different milk sources[J].Food Science,2012,33(5):58-61.
[21] ZHANG R H,PANG X Y,LU J,et al.Effect of high intensity ultrasound pretreatment on functional and structural properties of micellar casein concentrates[J].Ultrasonics Sonochemistry,2018,47:10-16.
