Food and Fermentation Industries >

2021 , Vol. 47 >Issue 15: 280 - 285

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026754

Determination of dihydroquercetin in food by solid phase extraction-high performance liquid chromatography

  • LIU Guiliang ,
  • LIAN Zhenxia ,
  • GONG Pixue ,
  • XUE Xia ,
  • LIU Yanming ,
  • ZHU Jianhua
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  • 1(Shandong Institute for Food and Drug Control,Jinan 250101,China)
    2(Shandong Research Center of Engineering and Technology for Safety Inspection of Food and Drug,Jinan 250101,China)
    3(Shandong Academy of Pharmaceutical Sciences,Jinan 250101,China)

Received date: 2021-01-14

  Revised date: 2021-02-18

  Online published: 2021-08-23

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Abstract

A novel method for the determination of dihydroquercetin in food by solid-phase extraction-high performance liquid chromatography was established. The sample was extracted by V(methanol)∶V(2% acetic acid-water)=1∶1 and purified by hydrophile-lipophile balance (HLB) solid-phase extraction column. Methanol-0.1% acetic acid water was used as a mobile phase for gradient elution. The sample was analyzed by HPLC and determined by a diode array detector. The retention time and spectrogram were qualitative and quantitative by the external standard method. The results showed that the linear range was 0.8-100.0 μg/mL and the linear correlation coefficient was 0.999 9. The detection limit reached 0.6 mg/kg and the quantitative limit was 2.0 mg/kg. Three concentration levels of 2, 20 and 200 mg/kg were added to beverage, fermented milk and cocoa products respectively. The average recoveries were between 90.3% and 102.5%, and the relative standard deviations were between 0.45% and 1.42%. The method has a good purification effect, high sensitivity and accuracy, and is suitable for the determination of dihydroquercetin in food.

Key words： dihydroquercetin; solid phase extraction; hydrophile-lipophile balance; high performance liquid chromatography; new food raw materials

Cite this article

LIU Guiliang , LIAN Zhenxia , GONG Pixue , XUE Xia , LIU Yanming , ZHU Jianhua . Determination of dihydroquercetin in food by solid phase extraction-high performance liquid chromatography[J]. Food and Fermentation Industries, 2021 , 47(15) : 280 -285 . DOI: 10.13995/j.cnki.11-1802/ts.026754

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