Food and Fermentation Industries >

2021 , Vol. 47 >Issue 16: 46 - 51

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.025983

Effect of high dose ingestion of hemp seed peptide and protein on renal function of mice

  • ZHU Yan ,
  • BI Yuan ,
  • WANG Yuchen ,
  • MA Yongqing ,
  • WANG Jing ,
  • XUE Chen ,
  • GU Ruizeng ,
  • WEI Ying
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  • (China National Research Institute of Food and Fermentation Industries Co. Ltd., Beijing Engineering Research Center of Protein & Functional Peptide Corporation Limited, Beijing 100015, China)

Received date: 2020-10-23

  Revised date: 2020-11-26

  Online published: 2021-09-10

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Abstract

The effect of high doses of hemp seed protein or hemp seed peptide on the renal function of normal or immunocompromised mice was studied in this paper. Low immunity animal model was established by intraperitoneal injection of 60 mg/kg cyclophosphamide for 3 d. The hemp seed protein and peptide were fed 10 g/kg for 30 d. Then the content of uric acid (UA), creatinine (CRE), blood urea nitrogen (BUN), immunoglobulin G (IgG) and immunoglobulin M (IgM) in serum were determined, also, the superoxide dismutase (SOD) and glutathion peroxidase (GSH-Px) activity and the content of malondialdehyde (MDA) in the kidney were measured by relevant kits, and the histopathological changes of the kidney in mice were observed by hematoxylin-eosin (HE) staining. Hemp seed protein and peptide had no significant effect on the contents of UN, CRE, BUN, IgG and IgM in serum, as well as the activity of SOD and GSH-Px and the content of MDA in the kidney in normal mice. And the HE staining results showed that these two did not cause damage to kidney tissue for normal mice. For low immunity in mice, hemp seed protein and peptide could reduce the UA, CRE, BUN content in serum, increased the content of serum IgG and IgM, and by increasing the SOD, GSH-Px activity and reducing MDA content in kidney to increase the kidney antioxidant capacity. Moreover, HE staining results also indicated that hemp seed protein and peptide could ease the degree of renal lesions caused by cyclophosphamide. Compared with hemp seed protein, the effect of hempseed peptide was stronger. Therefore, high dose of hemp seed protein and peptide had no damage to renal function in normal and immunocompromised mice, and had a certain effect of renal protection.

Key words： hempseed protein; hempseed peptide; renal function; cyclophosphamide

Cite this article

ZHU Yan , BI Yuan , WANG Yuchen , MA Yongqing , WANG Jing , XUE Chen , GU Ruizeng , WEI Ying . Effect of high dose ingestion of hemp seed peptide and protein on renal function of mice[J]. Food and Fermentation Industries, 2021 , 47(16) : 46 -51 . DOI: 10.13995/j.cnki.11-1802/ts.025983

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