Food and Fermentation Industries >

2021 , Vol. 47 >Issue 16: 78 - 83

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027475

Study on recovery of peptides from soybean proteolysis solution with epigallocatechin-3-gallate

  • WANG Jiayue ,
  • DONG Yabo ,
  • FU Yuantao ,
  • LAN Tian ,
  • SUI Xiaonan ,
  • WANG Huan ,
  • JIANG Lianzhou
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  • (College of Food Science, Northeast Agricultural University, Harbin 150030, China)

Received date: 2021-03-23

  Revised date: 2021-04-15

  Online published: 2021-09-10

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Abstract

The recovery of peptides from soy protein hydrolysates (SPHs) under different epigallocatechin-3-gallate (EGCG) concentrations and pH values were studied. The recovery rate of the peptide, the change of the secondary structure content, the surface hydrophobicity and the antioxidant activity were characterized. The results showed that increasing the enzymatic hydrolysis time could increase the intermediate peptide content. And the addition of EGCG also increased the recovery rate of SPHs. In addition, the amount of SPHs was positively correlated with the concentration of EGCG. With the increase of enzymatic hydrolysis time, the recovery rate of the peptide first tend to decrease and then increase, and the recovery rate was the highest at 30 min. It was indicated that EGCG might be easier to precipitate with peptides with a molecular weight of 5-10 kDa. To a certain extent, the addition of EGCG changed the secondary structure of the protein peptide, and the protein peptide was stretched. The addition of EGCG would also reduce the surface hydrophobicity of the SPHs and increase the antioxidant activity. This study provided a reference for EGCG to recover peptides from SPHs by constructing and analyzing the structure and function of SPHs-EGCG and its influence on the recovery rate of peptides.

Key words： epigallocatechin-3-gallate; soy peptides; peptide recovery rate; surface hydrophobicity; antioxidant activity

Cite this article

WANG Jiayue , DONG Yabo , FU Yuantao , LAN Tian , SUI Xiaonan , WANG Huan , JIANG Lianzhou . Study on recovery of peptides from soybean proteolysis solution with epigallocatechin-3-gallate[J]. Food and Fermentation Industries, 2021 , 47(16) : 78 -83 . DOI: 10.13995/j.cnki.11-1802/ts.027475

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