In order to explore the effects of reduced pressure treatment on fruit quality and physiology of postharvest litchi, ‘Feizixiao’ litchi was used as test material to study the effects of different reduced pressure treatments at 40, 60, 80 and 101.325 kPa (normal pressure) on fruit quality, cell membrane function and enzyme activities which related with mitochondrial respiratory metabolism of litchi fruit during normal temperature (25 ℃) shelf life. The results showed that compared with the control and other reduced pressure treatment conditions, 60 kPa reduced pressure treatment significantly inhibited the occurrence of browning and decay of litchi fruit. It also kept better freshness and quality of the fruit. After 60 kPa decompression treatment, litchi pericarp maintained cell membrane fluidity and retarded the increment of cell membrane permeability and maintained the integrity of cell membrane structure of litchi fruit during storage. Moreover, with the extension of postharvest storage time, 60 kPa reduced pressure treatment induced the increase of mitochondrial succinate dehydrogenase and cytochrome oxidase activities of litchi fruits during normal temperature shelf life, thus, kept activity of mitochondrion. Furthermore, 60 kPa reduced pressure treatment delayed the decreasing of adenosine triphosphate and adenosine diphosphate content. And it could also delay the increase of adenosine monophosphate content, inhibited the decrease of energy charge, and kept the pericarp of litchi at a higher energy level. These indexes were beneficial to maintain the quality of postharvest litchi during the shelf life at room temperature. Therefore, 60 kPa reduced pressure treatment is expected to be an effective measure for postharvest preservation of litchi fruit.
[1] 胡吉蕾, 雷艳, 刘青青, 等.川南晚熟荔枝保鲜过程中防止褐变的研究[J].食品与发酵科技, 2019, 55(3):35-38;49.
HU J L, LEI Y, LIU Q Q, et al.Research on preventing browning in the process of preserving late-ripening litchi in south of Sichuan[J].Food and Fermentation Technology, 2019, 55(3):35-38;49.
[2] 胡桂兵, 郑少泉, 向旭, 等.荔枝龙眼种业“十四五”发展思路[J].中国热带农业, 2020(3):4-6;16.
HU G B, ZHENG S Q, XIANG X, et al.Development of litchi and longan seed industry during the 14th Five-Year Plan[J].Tropical Agriculture of China, 2020(3):4-6;16.
[3] 刘亭, 钱政江, 杨恩, 等.呼吸活性和能量代谢与荔枝果实品质劣变的关系[J].果树学报, 2010, 27(6):946-951.
LIU T, QIAN Z J, YANG E, et al.Respiratory activity and energy metabolism of harvested litchi fruit and their relationship to quality deterioration[J].Journal of Fruit Science, 2010, 27(6):946-951.
[4] 郑先章, 郑郤, 熊伟勇.减压处理保鲜技术研究与应用前景[J].保鲜与加工, 2017, 17(3):124-128;132.
ZHENG X Z, ZHENG Y, XIONG W Y, et al.Research and application prospects on hypobaric treatment preservation technology[J].Preservation and Processing, 2017, 17(3):124-128;132.
[5] 王聘, 郜海燕, 周拥军, 等.减压处理对新疆白杏果实软化和细胞壁代谢的影响[J].农业工程学报, 2012, 28(16):254-258.
WANG P, GAO H Y, ZHOU Y J, et al.Effects of hypobaric storage on softening and cell wall metabolism of Xinjiang kuqa apricot fruits[J].Journal of Agricultural Engineering, 2012, 28(16):254-258.
[6] 陈文烜, 宋丽丽, 廖小军, 等.减压对水蜜桃采后呼吸途经和活性氧代谢的影响[J].中国食品学报, 2016, 16(7):170-175.
CHEN W Y, SONG L L, LIAO X J, et al.Effects of decompression on respiratory pathway and active oxygen metabolism of peaches after harvest[J].Chinese Journal of Food Science, 2016, 16(7):170-175.
[7] 王友升, 张萌, 王丹, 等.减压处理对草莓贮藏效果及活性氧代谢影响的多变量解析[J].中国食品学报, 2015, 15(6):231-239.
WANG Y S, ZHANG M, WANG D, et al.Effects of hypobaric storage on respiration pathway and active oxygen metabolism in juicy peach fruit[J].Acta Foodstuffs Sinica, 2015, 15(6):231-239.
[8] 林河通, 席玙芳, 陈绍军, 等.龙眼果实采后失水果皮褐变与活性氧及酚类代谢的关系[J].植物生理与分子生物学学报, 2005, 31(3):287-297.
LIN H T, XI Y F, CHEN S J, et al.The relationship between the desiccation-induced browning and the metabolism of active oxygen and phenolics in pericarp of postharvest Longan fruit[J].Acta Phytophysiology and Molecular Biology, 2005, 31(3):287-297.
[9] JIANG Y M, CHEN F.A study on polyamine change and-rowning of fruit during cold storage of litchi fruit[J].Postha-rvest Biology and Technology, 1995(5):245-250.
[10] JIANG Y M.Role of anthocyanins,polyphenol oxidase and phenols in lychee pericarp browning[J].Journal of the Science of Food and Agricultural, 2000, 80:305-310.
[11] 谭才邓. 植物线粒体提取的一种简捷方法[J].广东轻工职业技术学院学报, 2007(1):26-28;31.
TAN C D.A simple method for isolating mitochondrial from plant[J].Journal of Guangdong Light Industry Vocational and Technical College, 2007(1):26-28;31.
[12] ACKERLL B A, KEARNEY E B, SINGER T P.Mammalian succinate dehydrogenase[J].Methods Enzymol, 1978, 53:466-483.
[13] 朱广廉. 植物生理学实验[M].北京:北京大学出版社, 1990:34-37.
ZHU G L.Plant Physiology Experiment[M].Beijing:Peking University Press, 1990:34-37.
[14] ERREDE B, KAMEN M D.Comparative kinetic studies of cytochromes c in reactions with mitochondrial cytochrome c oxidase and reductase[J].Biochemistry, 1978, 17(6):1 015-1 027.
[15] OZOGUL F, QUANTICK P C, OZOGUL Y, et al.Arapid HPLC-determination of ATP-related compounds and its application to herring stored under modified atmosphere[J].International Journal of Food Science and Technology, 2000, 35:549-554.
[16] LIU H, JIANG Y, LUO Y, et al.A simple and rapid determination of ATP, ADP and AMP concentrations in pericarp tissue of litchi fruit by high performance liquid chromatography[J].Food Technology and Biotechnology, 2006, 44:531.
[17] 黄旭明, 王惠聪, 黄辉白, 等.易褐变材料荔枝果皮透射电镜制样固定方法的改进[J].植物生理学通讯, 2002(2):159-160.
HUANG X M, WANG H C, HUANG H B, et al.An improvement on the fixation of browning susceptible litchi pericarp samples for transmit electron microscopic observation[J].Plant Physiology Communications, 2002(2):159-160.
[18] 程志明, 李影, 王少峰, 等.荔枝采后果皮褐变机理研究[J].福建农业学报, 2005, 20(S1):77-79.
CHENG Z M, LI Y, WANG S F, et al.The mechanism of postharvest browning of litchi pericarp[J].Fujian Agricultural Journal, 2005, 20(S1):77-79.
[19] 许晓春, 吴振先, 陈维信, 等.荔枝果皮褐变机理研究进展[J].保鲜与加工, 2004, 4(1):8-10.
XU X C, WU Z X, CHEN W X, et al.Recent advances in research of pericarp browning of litchi[J].Preservation and Processing, 2004, 4(1):8-10.
[20] YANG B, JIANG Y M, CHEN F, et al.Extraction and pharmacological properties of bioactive compounds from longan (Dimocarpus longan Lour.) fruit—A review[J].Food Research International, 2011, 44(7):1 837-1 842.
[21] 熊海楠. 磷脂酶D在常温货架期间冷藏荔枝褐变过程中的作用[D].湛江:广东海洋大学, 2016.
XIONG H N.Effect of phospholipase D on browning of litchi fruit during shelf life at room temperature after cold storage[D].Zhanjiang:Guangdong Ocean University, 2016.