Sialyllactose has broad market prospects in infant formula industry. However, it is still a challenge to produce sialyllactose in large-scale. Escherichia coli JM109(DE3)/pET28a-neuA and E. coli JM109(DE3)/pET28a-nst were constructed to synthesize cytidine 5'-monophosphate N-acetylneuraminic acid (CMP-Neu5Ac) and 3'-sialyllactose, respectively. And then the optimized condition of CMP-Neu5Ac synthesis by E. coli JM109(DE3)/pET28a-neuA was developed. Finally, the whole-cell coupling fermentation method with beer yeast, E. coli JM109(DE3)/pET28a-neuA and E. coli JM109(DE3)/pET28a-nst was established to achieve 3'-sialyllactose. The CMP-Neu5Ac can be supplied and efficiently regenerated by the method, which will lay a forceful technical foundation for the economical and large-scale production of 3'-sialyllactose.
YOU Xing
,
GUO Jin
,
ZHANG Hongtao
,
YAN Jingyu
,
CHAI Wengang
,
ZHOU Wen
,
LIU Litong
,
LI Yu
,
WU Jianrong
,
ZHAN Xiaobei
. Synthesis of 3'-sialyllactose by coupling fermentation with different strains[J]. Food and Fermentation Industries, 2021
, 47(17)
: 8
-14
.
DOI: 10.13995/j.cnki.11-1802/ts.027472
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