Screening and identification of a dextranase-producing isolate and its enzyme characterization

  • ZU Hangtian ,
  • TIAN Xiaopeng ,
  • HU Jie ,
  • ZHANG Hao ,
  • DING Yanshuai ,
  • LYU Mingsheng ,
  • WANG Shujun
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  • 1(Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University,Lianyungang 222005,China)
    2(Co-Innovation Center of Jiangsu Marine Bio-industry Technology,Jiangsu Ocean University,Lianyungang 222000,China)

Received date: 2020-11-13

  Revised date: 2020-12-30

  Online published: 2021-09-27

Abstract

Dextranase plays an important role in food, medicine, and biotechnology industries. The dextranase-producing strain RN22 was isolated from marine mud in Rizhao Sea. It was identified as Pseudarthrobacter sp. base on colony morphology, physiological and biochemical characteristics, and 16S rRNA sequencing. Optimal fermentation conditions were as follows: 10 g/L of dextran T20, 5 g/L of peptone, 5 g/L of bran, pH 8.0, 30 ℃, and 48 h. The molecular mass of dextranase was about 70 kDa. The optimal temperature and pH were 60 ℃ and 7.5, respectively. The enzyme remained at 80% activity after incubated at 40 ℃ for 5 h and stable at pH 5.5-8.0. Mg2+, Na+, K+ could enhance its activity, and organic solvents could decrease its activity slightly. The enzyme could hydrolyze α-1,6 glycosidic specifically, and the content of maltotetraose, maltopentose and maltoheptose were over 90% in the hydrolysates. Dextranase would have promising applications in food and healthy industry base on its excellent property.

Cite this article

ZU Hangtian , TIAN Xiaopeng , HU Jie , ZHANG Hao , DING Yanshuai , LYU Mingsheng , WANG Shujun . Screening and identification of a dextranase-producing isolate and its enzyme characterization[J]. Food and Fermentation Industries, 2021 , 47(17) : 76 -83 . DOI: 10.13995/j.cnki.11-1802/ts.026165

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