Food and Fermentation Industries >

2021 , Vol. 47 >Issue 18: 127 - 134

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027739

Purification and immunomodulation activity of exopolysaccharides from Pleurotus ferulae Lanzi

  • WANG Jing ,
  • QIAO Jie ,
  • PEI Xinyun ,
  • CHANG Shimin ,
  • LIU Xuejuan ,
  • YAN Xunyou
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  • 1(College of Life Sciences, Langfang Normal University, Langfang 065000, China)
    2(Technical Innovation Center for Utilization of Edible and Medicinal Fungi in Hebei Province, Langfang 065000, China)
    3(Langfang Key Laboratory of Cell Engineering and Application, Langfang 065000, China)

Received date: 2021-04-21

  Revised date: 2021-05-13

  Online published: 2021-10-18

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Abstract

To investigate the optimum fermentation medium of exopolysaccharides from Pleurotus ferulae Lanzi, isolation and purification of water extract component in addition to its immunomodulation activity on macrophages and spleen lymphocytes were cultured in vitro. And ethanol precipitation, dialysis, lyophilization, DEAE-cellulose 52 chromatography and Sephadex G-100 chromatography were carried out to obtain water extract component of exopolysaccharides from P. ferulae Lanzi (PFEPw-1). The molecular weight and monosaccharide composition of PFEPw-1 were determined by high-performance gel permeation chromatography and high-performance liquid chromatography, respectively. Macrophages and spleen lymphocytes were isolated using the peritoneal lavage method and density gradient centrifugation, respectively. Fluorescent probe technique and enzyme-linked immunosorbent assay were carried out to measure the content of intracellular nitric oxide and reactive oxygen,and the levels of immune-related cytokines in culture supernatant,respectively.The results showed that PFEPw-1 was composed of D-mannose, D-glucose, D-galactose and L-arabinose L-fucose with a content ratio of 2.42∶3.90∶2.27∶1.41, and the molecular weight of PFEPw-1 was 64.2 kDa. Compared with the control group, 10-30 mg/L PFEPw-1 enhanced intracellular NO and reactive oxygen content (P<0.05),and improved the secretion level of tumor necrosis factor-α and interferon-γ in the culture supernatant (P<0.05) of macrophages cultured in vitro. Meanwhile, 20-30 mg/L PFEPw-1 could improve the secretion level of interleukin-1 and interleukin-6 in the culture supernatant of macrophages (P<0.05), and interleukin-2 and interferon-γ in the culture supernatant of spleen lymphocytes (P<0.05). The research provides theoretical support for industrial production, development and utilization of exopolysaccharides from P. ferulae Lanzi.

Key words： Pleurotus ferulae Lanzi; exopolysaccharides; macrophages; lymphocytes; immunomodulation

Cite this article

WANG Jing , QIAO Jie , PEI Xinyun , CHANG Shimin , LIU Xuejuan , YAN Xunyou . Purification and immunomodulation activity of exopolysaccharides from Pleurotus ferulae Lanzi[J]. Food and Fermentation Industries, 2021 , 47(18) : 127 -134 . DOI: 10.13995/j.cnki.11-1802/ts.027739

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