Food and Fermentation Industries >

2021 , Vol. 47 >Issue 18: 141 - 148

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027981

Protective effect of duck embryo-derived antioxidant peptide TD12 on oxidative stress damage in HepG2 cells

  • JI Zhengmei ,
  • ZHANG Xiaochun ,
  • PENG Yudi ,
  • WANG Shulin ,
  • BU Lijun ,
  • XIE Huadong
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  • 1(College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China)
    2(Chongqing Academy of Animal Sciences, Chongqing 402460, China)
    3(College of Biological Engineering, Chongqing University, Chongqing 400044, China)
    4(Chongqing Engineering Research Center of Meat Quality Evaluation and Processing, Chongqing 402460, China)

Received date: 2021-05-18

  Revised date: 2021-06-07

  Online published: 2021-10-18

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Abstract

This experiment aims to study the protective effect of duck embryo-derived anti-oxidant peptide TD12 on HepG2 cells from oxidative stress damage. The anti-oxidation activity of TD12 in vitro was evaluated by chemical assay. The cells apoptosis was observed by Hoechst 33342 staining. The cytotoxicity and protection of TD12 against oxidative damage were detected by CCK-8 method. And ROS, antioxidant enzyme activity and cytokine content were detected by DCFH-DA fluorescence probe, kit and ELISA method. The results showed that TD12 had a strong scavenging capacity of DPPH and ABTS hydroxyl radical, and had no toxic effect on HepG2 cells. And the accumulation of ROS in the cells was effectively decreased with the increase of TD12 concentration. The activities of SOD, CAT and GSH-Px were increased in a concentration-dependent manner. The TD12 could inhibit the release of LDH, MDA, TNF-ɑ and IL-8 in HepG2 cells injured by H2O2, and maintain the stability of IL-4 and IL-10. In conclusion, TD12 has a protective effect on oxidative stress injury in HepG2 cells.

Key words： duck embryo-derived antioxidant peptide TD12; oxidative stress; protective effects; reactive oxygen species; antioxidase active; enzyme linked immunosorbent assay

Cite this article

JI Zhengmei , ZHANG Xiaochun , PENG Yudi , WANG Shulin , BU Lijun , XIE Huadong . Protective effect of duck embryo-derived antioxidant peptide TD12 on oxidative stress damage in HepG2 cells[J]. Food and Fermentation Industries, 2021 , 47(18) : 141 -148 . DOI: 10.13995/j.cnki.11-1802/ts.027981

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