Food and Fermentation Industries >

2021 , Vol. 47 >Issue 18: 159 - 164

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027704

Effect of walnut green husk extracts on non-alcoholic fatty liver disease

  • FANG Qionglian ,
  • WANG Mengmeng ,
  • QIAO Xue ,
  • LI Xinping ,
  • HUANG Feng ,
  • LIN Yuping ,
  • ZHAO Shenglan
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  • (School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Resource, Yunnan University of Chinese Medicine, Kunming 650500, China)

Received date: 2021-04-15

  Revised date: 2021-05-14

  Online published: 2021-10-18

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Abstract

The effective components of walnut green husk were extracted by alcohol extraction, and different extracts were obtained by extraction with ethyl acetate and n-butanol. The effect of different extracts from walnut green husk on non-alcoholic fatty liver disease (NAFLD) were investigated. Specific pathogen free (SPF) male Sprague Dawley (SD) rats were randomly divided into normal control group, model group, positive control group and walnut green husk ethanol, ethyl acetate and n-butanol group. The rats were fed with high-fat diet for 12 weeks and given intragastric administration for 6 weeks. The serum levels of total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), glutamate aminotransferase (ALT) and aspartate aminotransferase (AST) were measured. The content of malondialdehyde (MDA), the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in liver tissue were checked. Pathological sections were analyzed by hematoxylin-eosin staining (HE). Compared with the normal group, the contents of TG, TC, HDL-C, ALT, AST and MDA in the model group were significantly increased (P<0.01), while the contents of LDL-C, GSH-Px and SOD activities were significantly decreased (P<0.001), and the pathological damage of liver tissue was obvious. Compared with the model group, the contents of TG, TC, HDL-C, ALT, AST and MDA in the ethanol, ethyl acetate and n-butanol extracts of walnut green husk decreased significantly (P<0.05), while the contents of LDL-C and the activities of GSH-Px and SOD increased significantly (P<0.01). Pathological damage was improved obviously. The n-butanol extract had more obvious effect than other extracts.

Key words： walnut green husk; NAFLD; HFD-induced; improving liver injury

Cite this article

FANG Qionglian , WANG Mengmeng , QIAO Xue , LI Xinping , HUANG Feng , LIN Yuping , ZHAO Shenglan . Effect of walnut green husk extracts on non-alcoholic fatty liver disease[J]. Food and Fermentation Industries, 2021 , 47(18) : 159 -164 . DOI: 10.13995/j.cnki.11-1802/ts.027704

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