In order to improve biomass yield of Bifidobacterium longum, three strains were determined when different nitrogen sources and trace elements were added to screening the optimum substrate. Furthermore, the osmotic pressure was measured when the growth rate of each strain was inhibited and growth completely inhibited. The results showed that B. longum had the highest utilization rate with yeast nitrogen. The limiting trace element was Mg2+. The substrate C/N ratio was based on the C/N consumption ratio when the strain growth rate was inhibited. The substrate concentration was based on the carbon and nitrogen consumption calculated by the theory of complete inhibition of osmotic pressure. In addition, the maximum biomass was positively correlated with Mg2+ in a certain range. When yeast extract FM803 was used as nitrogen source and glucose as carbon source (C/N mass ratio 2.5), the viable bacteria numbers of B. longum CCFM 687, CCFM 1029 and FGSZY 17L7 could reach to (7.9±1.1)×109, (9.3 ±0.5)×109, and (1.1 ±0.1)×1010 CFU/mL, 50-70 times higher than that of in MRS. The results will significantly improve the industrial production efficiency of B. longum.
GAO Xinwei
,
CUI Shumao
,
TANG Xin
,
MAO Bingyong
,
ZHAO Jianxin
,
CHEN Wei
. Optimization of substrate in high-density fermentation of Bifidobacterium longum[J]. Food and Fermentation Industries, 2021
, 47(19)
: 12
-20
.
DOI: 10.13995/j.cnki.11-1802/ts.025681
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