Various nitrogen source utilization in Bifidobacterium bifidum F35

  • WANG Wenli ,
  • CUI Shumao ,
  • TANG Xin ,
  • MAO Bingyong ,
  • ZHAO Jianxin ,
  • CHEN Wei
Expand
  • (School of Food Science and Technology,Jiangnan University,Wuxi 214122,China)

Received date: 2020-12-03

  Revised date: 2021-01-06

  Online published: 2021-11-04

Abstract

To study the features of nitrogen source utilization and mechanism of Bifidobacterium bifidum F35. The proliferation effects, the amino acid and peptide composition, the influence of amino acid nitrogen sources and peptide nitrogen sources on the proliferation, the amino acid transport and biosynthesis capacity of B. bifidum were analyzed. The results showed that B. bifidum used little yeast extract, preferring to use peptone; because B. bifidum used peptides more efficiently than amino acids, and the free amino acid of yeast extract were 2-5 times than that of other nitrogen sources, but the content of small molecule peptides below 2 000 Da were only half of others. The presence of a large number of hydrophobic amino acids would inhibit the growth of strains, but not affect the strain's utilization of polypeptides; it had the ability to synthesize 17 amino acids and rich amino acid transport system. Its genotype and phenotype were both cysteine auxotroph. The results provide theoretical guidance for the future production of nitrogen sources related to B.bifidum

Cite this article

WANG Wenli , CUI Shumao , TANG Xin , MAO Bingyong , ZHAO Jianxin , CHEN Wei . Various nitrogen source utilization in Bifidobacterium bifidum F35[J]. Food and Fermentation Industries, 2021 , 47(19) : 21 -28 . DOI: 10.13995/j.cnki.11-1802/ts.026359

References

[1] 李吉平,陈雪,刘建华,等.双歧杆菌生物特性及其功能研究进展[J].中国奶牛,2020(6):57-61.
LI J P,CHEN X,LIU J H,et al.Advances in biological characteristics and functions of Bifidobacterium[J].China Dairy Cattle,2020(6):57-61
[2] 杜越欧,侯俊财.乳酸菌蛋白水解体系及相关基因表达的研究进展[J].食品工业科技,2013,34(3):383-386;391.
DU Y O,HOU J C.Research progress in proteolysis system of lactic acid bacteria and related gene expression[J].Science and Technology of Food Industry,2013,34(3):383-386;391.
[3] BROWN L,PINGITORE E V,MOZZI F,et al.Lactic acid bacteria as cell factories for the generation of bioactive peptides[J].Protein and Peptide Letters,2017,24(2):146-155.
[4] TURRONI F,DURANTI S,BOTTACINI F,et al.Bifidobacterium bifidum as an example of a specialized human gut commensal[J].Frontiers in Microbiology,2014,5:437.
[5] JANER C,ARIGONI F,LEE B H,et al.Enzymatic ability of Bifidobacterium animalis subsp. lactis to hydrolyze milk proteins:Identification and characterization of endopeptidase O[J].Applied & Environmental Microbiology,2005,71(12):8 460-8 465.
[6] CHANG O K,SEOL K H,JEONG S G,et al.Casein hydrolysis by Bifidobacterium longum KACC91563 and antioxidant activities of peptides derived therefrom[J].Journal of Dairy Science,2013,96(9):5 544-5 555.
[7] 王靖麟,王世博,徐睿,等.鲟鱼肝酶解条件优化及酶解液对双歧杆菌增殖效果的研究[J].中国酿造,2020,39(4):152-158.
WANG J L,WANG S B,XU R,et al.Optimization of enzymatic hydrolysis conditions of sturgeon liver protein and effect of the hydrolysate on Bifidobacterium proliferation[J].China Brewing,2020,39(4):152-158.
[8] 张娜,郭庆启,石彦国,等.促进益生菌增殖的大豆蛋白水解物的制备方法及其应用: CN107912533A[P].2018-04-17.
ZHANG N,GUO QQ,SHI Y G,et al.Preparation method and application of soybean protein hydrolysate for promoting probiotics proliferation: China CN107912533A[P].2018-04-17.
[9] 周兴雅.格氏乳杆菌与副格氏乳杆菌的筛选,基因组比较及安全性评价[D].无锡:江南大学,2019.
ZHOU X Y.Isolation,comparative genomics and safety assessment of Lactobcillus gasseri and Lactobaacillus paragasseri[D].Wuxi:Jiangnan University,2019.
[10] 赵杰,岳华,苟学磊,等.伊枯草菌素A发酵过程中游离氨基酸的HPLC分析[J].生物技术通报,2018,34(8):151-158.
ZHAO J,YUE H,GOU X L,et al.Analysis of free amino acids during fermentation of iturin a by high performance liquid chromatography[J].Biotechnology Bulletin,2018,34(8):151-158.
[11] FERRARIO C,DURANTI S,MILANI C,et al.Exploring amino acid auxotrophy in Bifidobacterium bifidum PRL2010[J].Frontiers in Microbiology,2015,6:1331.
[12] CRONIN M,ZOMER A,FITZGERALD G,et al.Identification of iron-regulated genes of Bifidobacterium breve UCC2003 as a basis for controlled gene expression[J].Bioengineered Bugs,2012,3(3):157-167.
[13] BROWN J,PIRRUNG M,MCCUE L A.FQC Dashboard:Integrates FastQC results into a web-based,interactive,and extensible FASTQ quality control tool[J].Bioinformatics,2017,33(19):3 137-3 139.
[14] DELCHER A L,BRATKE K A,POWERS E C,et al.Identifying bacterial genes and endosymbiont DNA with Glimmer[J].Bioinformatics,2007,23(6):673-679.
[15] BESEMER J,BORODOVSKY M.GeneMark:web software for gene finding in prokaryotes,eukaryotes and viruses[J].Nucleic Acids Research,2005,33(suppl_2):W451-W454.
[16] LOWE T M,CHAN P P.tRNAscan-SE On-line:Integrating search and context for analysis of transfer RNA genes[J].Nucleic Acids Research,2016,44(W1):W54-W57.
[17] BLEASBY A J,AKRIGG D,ATTWOOD T K.OWL:a non-redundant composite protein sequence database[J].Nucleic Acids Research,1994,22(17):3 574-3 577.
[18] BAIROCH A,APWEILER R.The SWISS-PROT protein sequence database and its supplement TrEMBL in 2000[J].Nucleic Acids Research,2000,28(1):45-48.
[19] OGATA H,GOTO S,SATO K,et al.KEGG:Kyoto encyclopedia of genes and genomes[J].Nucleic Acids Research,2000,28(1):27-30.
[20] RIBARDO D A,HENDRIXSON D R.Analysis of the LIV system of Campylobacter jejuni reveals alternative roles for LivJ and LivK in commensalism beyond branched-chain amino acid transport.[J].Journal of Bacteriology,2011,193(22):6 233-6 243.
[21] KAISER J C,OMER S,SHELDON J R,et al.Role of BrnQ1 and BrnQ2 in branched-chain amino acid transport and virulence in Staphylococcus aureus[J].Infection and Immunity,2015,83(3):1 019-1 029.
[22] DEN HENGST C D,GROENEVELD M,KUIPERS O P,et al.Identification and functional characterization of the Lactococcus lactis CodY-regulated branched-chain amino acid permease BcaP (CtrA)[J].Journal of Bacteriology,2006,188(9):3 280-3 289.
[23] KREDICH H M.Biosynthesis of cysteine[J].Ecosal Plus,2008,3(1).DOI:10.1128/ecosalplus.3.6.1.11
[24] ST-GELAIS D,ROY D,HACHÉ S,et al.Growth of nonproteolytic Lactococcus lactis in culture medium supplemented with different casein hydrolyzates[J].Journal of Dairy Science,1993,76(11):3 327-3 337.
[25] 张清丽.酪蛋白活性肽对乳酸菌生长代谢及酸乳发酵影响的研究[D].广州:华南理工大学,2011.
ZHANG Q L.Studies on the infuence of casein-derived bioactive peptides on lactic acid bacteria metabolism and yoghurt fermentation[D].Guangzhou:South China University of Technology,2011.
[26] PROULX M,WARD P,GAUTHIER S F,et al.Comparison of Bifidobacterial growth-promoting activity of ultrafiltered casein hydrolyzate fractions[J].Le Lait,1994,74(2):139-152.
[27] KONGO J M,GOMES A M P,MALCATA F X.Development of a chemically defined medium for growth of Bifidobacterium animalis[J].Journal of Food Science,2002,68(9):2 742-2 746.
[28] AL-YOUNES H M,BRINKMANN V,MEYER T F.Interaction of Chlamydia trachomatis serovar L2 with the host autophagic pathway[J].Infection and Immunity,2004,72(8):4 751-4 762.
[29] BRAUN P R,AL-YOUNES H,GUSSMANN J,et al.Competitive inhibition of amino acid uptake suppresses chlamydial growth:Involvement of the chlamydial amino acid transporter BrnQ[J].Journal of Bacteriology,2008,190(5):1 822-1 830.
[30] STUCKY K,HAGTING A,KLEIN J R,et al.Cloning and characterization of brnQ,a gene encoding a low-affinity,branched-chain amino acid carrier in Lactobacillus delbrdückii subsp.lactic DSM-7290[J].Molecular and General Genetics MGG,1995,249(6):682-690.
[31] LANGE C,RITTMANN D,WENDISCH V F,et al.Global expression profiling and physiological characterization of Corynebacterium glutamicum grown in the presence of L-valine[J].Applied and Environmental Microbiology,2003,69(5):2 521-2 532.
Outlines

/