Food and Fermentation Industries >

2021 , Vol. 47 >Issue 19: 63 - 70

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026400

Strain breeding for high-yielding α-cyclodextrin glucosyltransferase and optimization of the enzyme formation conditions

  • TAO Dawei ,
  • ZHANG Xiaodan ,
  • NING Xibin ,
  • SUN Mengjie
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  • 1(College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China)
    2(Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation,Shanghai 201306,China)
    3(Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture,Shanghai 201306,China)
    4(National R&D Branch Center for Freshwater Aquatic Products Processing Technology (Shanghai),Shanghai 201306,China)

Received date: 2020-12-07

  Revised date: 2020-12-30

  Online published: 2021-11-04

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Abstract

Paenibacillus macerans TLLY7 was mutated by irradiation with 20 W UV lamp for 70 s and microwave irradiation at low power for 60 s, and a high-production α-cyclodextrin glucosyltransferase (α-CGTase) strain UM2-6 was obtained with stable genetic performance. The results showed that the optimal fermentation culture medium were 10 g/L of soluble starch, 10 g/L of yeast powder, 2 g/L of NaCl, 0.2 g/L of CaCl2 and 1 g/L of K2HPO4·3H2O. The optimal fermentation conditions were 37.1 ℃, initial pH 6.9 and inoculum volume 4%. Strain UM2-6 produced 4.19 U/mL α-CGTase, which was 1.36 times than that of before optimization (3.09 U/mL). This study provides a reference for further industrial development using strains with stable and high production of α-CGTase.

Key words： compound mutation; ultraviolet mutagenesis; microwave mutagenesis; α-cyclodextrin glucosyltransferase; optimization of fermentation conditions

Cite this article

TAO Dawei , ZHANG Xiaodan , NING Xibin , SUN Mengjie . Strain breeding for high-yielding α-cyclodextrin glucosyltransferase and optimization of the enzyme formation conditions[J]. Food and Fermentation Industries, 2021 , 47(19) : 63 -70 . DOI: 10.13995/j.cnki.11-1802/ts.026400

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